Table 3 Detection limits of real-time PCR assay for spiked porcine DNA in cosmetic samples
Cosmetics | Extraction methods | Threshold cycle (Ct) | ||||
|---|---|---|---|---|---|---|
2.28 × 104 (copies/tube) | 2.28 × 103 (copies/tube) | 2.28 × 102 (copies/tube) | 2.28 × 101 (copies/tube) | 2.28 × 100 (copies/tube) | ||
Liquid-type mask pack | CTAB | 27.34 ± 0.60a | 31.18 ± 0.55 | 35.06 ± 0.21 | No Ct | No Ct |
Power Prep™ DNA extraction kit | 24.28 ± 0.19 | 27.44 ± 0.39 | 30.97 ± 0.15 | 35.21 ± 0.21 | 38.51 ± 0.30 | |
QIAamp DNA stool mini kit | 26.16 ± 0.16 | 30.49 ± 0.56 | 34.06 ± 0.15 | 38.37 ± 0.23 | No Ct | |
Wizard genomic DNA purification kit | 30.56 ± 0.47 | 33.64 ± 0.33 | 37.76 ± 0.79 | No Ct | No Ct | |
TIANamp genomic DNA Kit | 24.99 ± 0.21 | 28.19 ± 0.11 | 32.07 ± 0.41 | 35.77 ± 0.49 | 38.71 ± 0.61 | |
Nucleo spin food kit | 27.19 ± 0.21 | 31.00 ± 0.11 | 35.22 ± 0.24 | 38.94 ± 0.21 | No Ct | |
Powder-type mask pack | CTAB | 30.11 ± 0.11 | 33.86 ± 0.17 | 38.13 ± 0.14 | No Ct | No Ct |
Power Prep™ DNA extraction kit | 27.56 ± 0.24 | 31.94 ± 0.08 | 35.48 ± 0.37 | 39.07 ± 0.09 | No Ct | |
QIAamp DNA stool mini kit | 26.63 ± 0.50 | 29.75 ± 0.67 | 34.14 ± 0.86 | 37.63 ± 0.97 | No Ct | |
Wizard genomic DNA purification Kit | 34.14 ± 0.34 | 38.75 ± 0.40 | No Ct | No Ct | No Ct | |
TIANamp genomic DNA Kit | 30.59 ± 1.28 | 34.04 ± 1.26 | 38.42 ± 1.56 | No Ct | No Ct | |
Nucleo spin food kit | 30.40 ± 0.68 | 33.67 ± 0.22 | 36.73 ± 0.34 | No Ct | No Ct | |
Cream | CTAB | 30.57 ± 0.72 | 34.69 ± 0.09 | 38.52 ± 0.52 | No Ct | No Ct |
Power Prep™ DNA extraction kit | 26.41 ± 0.28 | 30.16 ± 0.23 | 34.03 ± 0.27 | 36.78 ± 0.34 | 39.07 ± 0.07 | |
QIAamp DNA stool mini kit | 30.97 ± 0.70 | 34.35 ± 1.22 | No Ct | No Ct | No Ct | |
Wizard genomic DNA purification Kit | 29.96 ± 0.16 | 34.44 ± 0.38 | 38.7 ± 0.33 | No Ct | No Ct | |
TIANamp Genomic DNA Kit | 29.52 ± 0.98 | 33.50 ± 1.20 | 38.47 ± 1.12 | No Ct | No Ct | |
Nucleo spin food kit | 29.02 ± 0.09 | 33.15 ± 0.20 | 36.8 ± 0.33 | No Ct | No Ct | |