Evaluation of polyembryony for genetic resources and efficacy of simple sequence repeat markers for the identification of nucellar and zygotic embryo-derived individuals in citrus

Woo, Jin-kyu; Park, Young Chul; Lee, Ju Won; Yun, Su-Hyun; Kim, Minju; Park, Sukman; Lee, Yi; Song, Kwan Jeong; Kim, Ho Bang

doi:10.1186/s13765-019-0437-1

Applied Biological Chemistry

Table 2 Information on 17 polymorphic SSR markers selected finally from the screening

From: Evaluation of polyembryony for genetic resources and efficacy of simple sequence repeat markers for the identification of nucellar and zygotic embryo-derived individuals in citrus

Marker	Previous name	Primer sequence (5′ → 3′)	Repeat motif	Ta (°C)^a	Size range (bp)
BM-CiSSR-012	CiBE0914^b	GGGCTCAGTTCTTCTCTACTC	(TTA)15	58	283–308
BM-CiSSR-012	CiBE0914^b	GCATTAGGCTTCTCTCATACC	(TTA)15	58	283–308
BM-CiSSR-013	CiBE1098	GGTGGCATACATACATACATACA	(TA)6	58	127–175
BM-CiSSR-013	CiBE1098	GCAACATCTGGAACTACTCA	(TA)6	58	127–175
BM-CiSSR-032	CiBE2380	GCCTGAGTTTCTTTGTTATG	(TATG)4	58	137–188
BM-CiSSR-032	CiBE2380	CATTCCATCGTCTCCTATTGT	(TATG)4	58	137–188
BM-CiSSR-043	CiBE3458	ATTAGTGCGGGTAAGATGAA	(AAAAT)3	58	261–332
BM-CiSSR-043	CiBE3458	AAGGATTTGGTGTAGGAAGTAA	(AAAAT)3	58	261–332
BM-CiSSR-073	CiBE6092	CGGACAAGGAGATGAAGATAG	(GA)16	58	319–345
BM-CiSSR-073	CiBE6092	TTCTAACAGCACCAAGCAG	(GA)16	58	319–345
BM-CiSSR-077	CiBE6256	TGTATTTATTTCTGACTACGACC	(AT)12	58	182–207
BM-CiSSR-077	CiBE6256	ATGCGTTTGGTGTGTGTT	(AT)12	58	182–207
BM-CiSSR-082	Luro0016^c	ACCTGAGCCCTTTTTGGTTT	(TC)13	58	145–158
BM-CiSSR-082	Luro0016^c	GCCAGATCAAGGCTCAAATC	(TC)13	58	145–158
BM-CiSSR-087	Luro0032	CAGATCCTATTGCAGAGGCA	(CAG)6	58	183–192
BM-CiSSR-087	Luro0032	GCCCATTTGTATTGCCATTT	(CAG)6	58	183–192
BM-CiSSR-093	Luro0115	CCCCCTCTTCTTTCACACAA	(TA)6	58	145–166
BM-CiSSR-093	Luro0115	GGTGAGCAGCCATCTTCTTC	(TA)6	58	145–166
BM-CiSSR-094	Luro0116	GAATTGGGAGGACGAACTGA	(AGA)7	58	265–277
BM-CiSSR-094	Luro0116	CGAGCCCTAGACAGAGATGG	(AGA)7	58	265–277
BM-CiSSR-100	Luro0164	GTTTTCAGCTGGATTCGAGG	(GCC)5	58	192–206
BM-CiSSR-100	Luro0164	CACGTGTCCTCCTGGAACTT	(GCC)5	58	192–206
BM-CiSSR-111	Luro0430	CCGATACAGCACAAAGCAAA	(AAT)7N15(AGC)7	58	134–147
BM-CiSSR-111	Luro0430	TGGAAAGAGAGAAGCCAAGC	(AAT)7N15(AGC)7	58	134–147
BM-CiSSR-115b	Luro0817	CGGTGTGTATTGGGTACACG	(TA)17	58	231–255
BM-CiSSR-115b	Luro0817	GCTTTTTCGAAAGCGTCAAG	(TA)17	58	231–255
BM-CiSSR-137	In this study	GCAACGTGTACTGACGCTTG	(TAT)7	53	292–330
BM-CiSSR-137	In this study	GCTCGTATCTGAAGCTCGCC	(TAT)7	53	292–330
BM-CiSSR-159	In this study	ATGACCTCAAACGGTGAGCA	(GAGG)5	53	378–404
BM-CiSSR-159	In this study	CTTCCACATCCGAACCGACA	(GAGG)5	53	378–404
BM-CiSSR-162	In this study	GCTAGGGTTCCAGACTTCCAG	(AAT)10(CAT)6	53	180–210
BM-CiSSR-162	In this study	GATTTGGCCGATCGAAAGCC	(AAT)10(CAT)6	53	180–210
BM-CiSSR-165	In this study	AGCAACTTAAGGTCCTTCACGA	(AAT)6	53	393–448
BM-CiSSR-165	In this study	TTCTCTGCTCTGCTGTGCAT	(AAT)6	53	393–448

^aTa means annealing temperature. ^bInformation of SSR markers starting with CiBE was obtained from Ollitrault et al. [16]. ^cSSR markers starting with Luro were obtained from Luro et al. [15]

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