Physiological and proteomic analyses of Korean F1 maize (Zea mays L.) hybrids under water-deficit stress during flowering

Kim, Sang Gon; Lee, Jin-Seok; Bae, Hwan Hee; Kim, Jung-Tae; Son, Beom-Young; Kim, Sun-Lim; Baek, Seong-Bum; Shin, Seonghyu; Jeon, Weon-Tai

doi:10.1186/s13765-019-0438-0

Applied Biological Chemistry

Table 1 Differentially accumulated proteins identified in drought-stressed plants of Korean F1 maize hybrids, Ilmichal (Ilmi) and Gwangpyeongok (GPOK), using MALDI–TOF MS

From: Physiological and proteomic analyses of Korean F1 maize (Zea mays L.) hybrids under water-deficit stress during flowering

No.	Description	ID (UniProt)	Mr/pI^a (theoretical)	Score	MP^b	SC^c (%)	Fold change		Biological function
No.	Description	ID (UniProt)	Mr/pI^a (theoretical)	Score	MP^b	SC^c (%)	Ilmi	GPOK	Biological function
1	Uncharacterized protein	B4FD04	11,794/6.60	116	9	48	− 1.84	− 1.63	Unknown
2	Small heat shock protein	Q41815	26,302/7.88	171	20	52	+ 2.25	+ 2.67	Stress response
3	Glutathione transferase 5	O24595	25,445/6.21	103	9	34	+ 3.34	+ 4.26	Stress response
4	Uncharacterized protein	B4G0G1	22,054/5.90	137	13	42	+ 2.68	+ 3.05	Unknown
5	17.4 kDa class I heat shock protein 3	B6TDB5	17,735/5.36	246	15	57	+ 1.79	+ 2.11	Stress response
6	Ascorbate peroxidase	C3VQ48	27,751/6.07	115	7	35	+ 1.52	+ 1.64	Stress response
7	60S ribosomal protein L24	B6TH56	23,901/9.80	85	9	28	− 1.80	− 1.96	Protein synthesis
8	Delta 3,5-delta 2,4-dienoyl-CoA isomerase	B4FD65	30,492/8.21	90	11	26	–	+ 2.47	Fatty acid catabolic metabolism
9	Malate dehydrogenase	F6MFD6	36,704/5.05	108	8	25	+ 3.05	+ 4.25	Carbohydrate metabolism
10	Cysteine proteinase inhibitor	Q4FZ52	26,941/6.11	230	12	45	+ 5.72	+ 6.28	Stress response
11	Triosephosphate isomerase	P12863	27,236/5.52	165	13	59	− 5.36	− 7.49	Carbohydrate metabolism
12	Oxygen evolving enhancer protein 3	B6SP64	25,555/7.66	162	12	45	− 2.23	− 1.68	Photosynthesis
13	NAD-dependent epimerase/dehydratase	B4FH62	31,865/6.99	129	8	40	+ 1.58	–	Carbohydrate metabolism
14	NAD-dependent epimerase/dehydratase	B6T962	27,795/6.77	256	15	61	+ 2.30	+ 1.96	Carbohydrate metabolism
15	Serine/threonine-protein phosphatase PP1	P22198	36,424/5.27	143	10	31	+ 1.95	+ 1.53	Stress response
16	Carbonic anhydrase	B4FWC5	33,817/7.11	123	11	27	− 2.16	− 1.83	Carbohydrate metabolism
17	Peroxidase 66	A5H454	33,912/8.39	194	19	55	+ 3.81	+ 4.36	Stress response
18	Fructose-bisphosphate aldolase	P08440	39,036/7.52	234	16	41	− 1.64	− 2.11	Carbohydrate metabolism
19	Uncharacterized protein	B4F844	36,280/5.88	155	13	36	+ 1.67	–	Unknown
20	Alpha-galactosidase	B4F9X0	45,527/5.63	170	16	35	+ 2.44	+ 1.83	Carbohydrate metabolism
21	Glyceraldehyde-3-phosphate dehydrogenase	B4F8L7	47,663/5.95	237	18	35	− 4.45	− 6.78	Carbohydrate metabolism
22	Isocitrate dehydrogenase	K7V067	46,636/6.54	123	17	26	+ 2.45	+ 1.69	Carbohydrate metabolism
23	Bifunctional 3-phosphoadenosine 5-phosphosulfate synthetase 2	B6SRJ5	52,493/8.30	190	23	47	–	− 2.26	Energy metabolism
24	NAD(P)H-quinone oxidoreductase subunit 2 A	P0CD58	57,521/5.23	185	22	25	+ 1.63	–	Photosynthesis

^aTheoretical molecular weight/theoretical isoelectric point
^bNumber of matched peptides
^cSequence coverage (%)

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