Table 1 Primers and plasmids used in this study
Description | Source or reference | |
|---|---|---|
Primers | ||
CotB-F | CGACGCGTGTATATTAAAAACCGTTCAC | This study |
CBGP-R | GCTCACCATATGATAGTCTATCGTTCTGACAATCG | This study |
CoP-R | CTCGCCCTTGCTCACCATATGATTGCTGTCCTTATCATTATTGTCTTC | This study |
CBGP-F | ATAGACTATCATATGGTGAGCAAGGGCGAG | This study |
GFP-R | ACTAGCTAGCCATTATAAGCTGCA | This study |
CoP-F | GAAGACAATAATGATAAGGACAGCAATCATATGGTGAGCAAGGGCGAG | This study |
Plasmids | ||
pUC19 | E. coli vector, Apr | Lab collection |
pUB110 | B. subtilis vector, Kmr | Lab collection |
pEGFP-N1 | Eukaryotic expression vector carrying gfp | Clontech |
pTOP-V2 | E. coli cloning vector, Apr, Kmr | Enzynomics |
pHY300PLK | E. coli-B. subtilis shuttle vector, Apr, Tcr | Takara Bio. Co., Ltd. |
pUB19 | E. coli-B. subtilis shuttle vector, Apr, Kmr | This study |
pTOP-V2-cotB-gfp | cloning of cotB-gfp fusion gene | This study |
pTOP-V2-ΔcotB-gfp | Cloning of ΔcotB-gfp fusion gene | This study |
pUB19-cotB-gfp | Spore display of GFP using CotB anchor | This study |
pUB19-ΔcotB-gfp | Spore display of GFP using ΔCotB anchor | This study |
pHY300-cotB-gfp | Spore display of GFP using CotB anchor | This study |
pHY300-ΔcotB-gfp | Spore display of GFP using ΔCotB anchor | This study |