Fig. 5

NAC-dependent elimination of carnosol-induced ROS generation. Cells were treated with NAC (5 mM) for 12 h with or without carnosol treatment (100 μM), then ROS levels were measured by fluorescence microscopy (a) or by flow cytometry (b) after DCF-DA staining. c The percent intensity of ROS generation compared to non-stained blank was calculated using geomean value and plotted as a graph. The statistical significance was determined by Student’s T-test against non-treated control. *p < 0.01 compared to control group