Low-density lipoprotein-antioxidant flavonoids and a phenolic ester from Plectranthus hadiensis var. tomentosus

Applied Biological Chemistry

Table 4 Quantitative analysis of compounds 1, 2, and 7 in PHT using HPLC–DAD

Compounds	Linear regression data				LOD (µg/mL)	LOQ (µg/mL)
Compounds	Calibration curve	R²	SD	RSD%	LOD (µg/mL)	LOQ (µg/mL)
1	y = 5814.468x + 6319.507	0.9997	108.74	1.84	0.06	0.19
2	y = 4100.056x + 7260.455	0.9993	84.70	2.02	0.07	0.21
7	y = 10,254.18x + 22,178.86	0.9986	293.48	2.76	0.09	0.29

Values indicate mean ± SD from two independent experiments performed in triplicate. The limit of detection (LOD) and quantification (LOQ) were determined according to the International Conference of Harmonization (ICH)-Q2 guidelines (ICH. 2005). LOD = 3.3 δ/S and LOQ = 10 δ/S, where δ = SD of the response and S = slope of the calibration curve