Fig. 2
From: FLOWERING HTH1 is involved in CONSTANS-mediated flowering regulation in Arabidopsis
Subcellular and vasculature expression of FHTH1. a Nuclear localization of CO-GFP and FHTH1-GFP in tobacco (Nicotiana benthamiana) leaf epidermal cells. Scale bars = 50 μm. b In planta interaction between FHTH1 and CO. The N-terminal fragment of Venus (VN) and C-terminal fragment of Venus (VC) were fused to FHTH1 and CO, respectively. Constructs constitutively expressing VN-FHTH1 and VC-CO were introduced into 3-week-old N. benthamiana leaves. Scale bar = 100 μm. c FHTH1 expression pattern in the FHTH1:GUS transgenic plant. A 2.0 kb FHTH1 promoter fragment was cloned in front of the GUS coding sequence. The transgenic plants were grown for 10 days in long days. A detached cotyledon (left panel) and first true leaf (right panel) are shown. FHTH1:GUS activity was detected in the leaf vasculature. Scale bars = 200 μm