Fig. 3

Identification and flowering time of the fhth1 mutant. a The position of the T-DNA insertion in the FHTH1 locus. The T-DNA was located in the first intron of the FHTH1 gene. Black boxes, lines, and white boxes represent exons, introns, and UTRs, respectively. b Genomic PCR (left panel) and RT-PCR (right panel) to identify the fhth1 mutant. We characterized the homozygous mutant using combinations of the FHTH1 left primer (LP), right primer (RP), and T-DNA-specific left board primer (LBb1) for genomic PCR and comparing FHTH1 expression levels between wild-type and mutant plants. ACTIN2 was used as an internal control. c, d Flowering time of fhth1 plants grown in long days. d The number of rosette leaves were counted on the main stem. Mean ± SD