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Applied Biological Chemistry
Fig. 3 | Applied Biological Chemistry

Fig. 3

From: Methyl lucidone exhibits neuroprotective effects on glutamate-induced oxidative stress in HT-22 cells via Nrf-2/HO-1 signaling

Fig. 3

MLC increases HO-1 expression and Nrf-2 activity. a After pretreating with MLC (5 μM) for 1 h, the cells were washed and further incubated in fresh media for 2, 5, 11 or 23 h. The expression of HO-1 was determined by Western blot analysis. *p < 0.05 and **p < 0.01, compared to the untreated group (0 h MLC). b After treating MLC for 10 min, 30 min, 1 h and 2 h, the nuclei of HT-22 cells were fractionated from the cytosol by using NE-PER Nuclear and Cytoplasmic Extraction Reagents as described in Materials and methods. Nrf-2 proteins were detected by Western blot analysis (n = 3). **p < 0.01, compared to the untreated group (0 min MLC). The data indicate mean ± SEM

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