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Applied Biological Chemistry
Fig. 4 | Applied Biological Chemistry

Fig. 4

From: Methyl lucidone exhibits neuroprotective effects on glutamate-induced oxidative stress in HT-22 cells via Nrf-2/HO-1 signaling

Fig. 4

The antioxidant effects of MLC are blocked by HO-1 inhibition. HT-22 cells were pretreated with SnPP (20 μM) for 2 h before treating with MLC. After pretreating with MLC (1, 3, 5 μM) for 1 h, these cells were washed and incubated with glutamate (5 mM) for 12 h. a The cell viability was measured by using MTT (2 mg/ml) solution. **p < 0.01, compared to the untreated group (no drugs). ##p < 0.01, compared to the group treated with glutamate alone. ††p < 0.01, indicating the significance with or without SnPP pretreatment in groups treated with MLC and glutamate. b Intracellular ROS levels were measured using ROS-sensitive fluorescent DCF-DA. **p < 0.01, compared to the untreated group (no drugs). #p < 0.05, compared to the group treated with glutamate alone. †p < 0.05, indicating the significance with or without SnPP pretreatment in groups treated with MLC and glutamate. The data indicate mean ± SEM (n = 3)

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