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Applied Biological Chemistry
Fig. 6 | Applied Biological Chemistry

Fig. 6

From: Methyl lucidone exhibits neuroprotective effects on glutamate-induced oxidative stress in HT-22 cells via Nrf-2/HO-1 signaling

Fig. 6

MLC protects HT-22 neurons via activating PI3K/Akt pathway. HT-22 cells were pretreated with LY294002 (20 μM) for 1 h before treating with MLC. After pretreating with MLC (5 μM) for 1 h, these cells were washed and incubated with glutamate (5 mM) for 5 h or 12 h for measuring HO-1 expression or cell viability, respectively. a Changes of HO-1 protein expression detected by Western blot analysis. *p < 0.05 or **p < 0.01, compared to the untreated group (no drugs). #p < 0.05, indicating the significant with or without LY294002 in the group treated with MLC. b Changes of Nrf-2 expression. **p < 0.01, compared to the untreated group (no drugs). ##p < 0.01, compared to the MLC-treated group. c Changes of cell viability measured by MTT assay. *p < 0.01, compared to the untreated group (no drugs), ##p < 0.01, compared to the group treated with glutamate alone. ††p < 0.01, indicating the significance with or without LY294002 pretreatment in groups treated with MLC and glutamate. The data indicate mean ± SEM (n = 3)

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