Expression of Jerusalem artichoke (Helianthus tuberosus L.) fructosyltransferases, and high fructan accumulation in potato tubers

Applied Biological Chemistry

Table 1 Primers used for reverse transcription PCR and quantitative real-time PCR (qRT-PCR) of fructosyltransferases and actin

Gene name	GenBank accession number	Primer name	Primer sequence (5′ → 3′)	Fragment size (bp)
Actin	XM_006345899	Actin_F	GTATTGTGTTGGACTCTGGTG	614
		Actin_R	CTGCTGGAAGGTGCTGAGGGA	614
		Actin_QF	GCTTCCCGATGGTCAAGTCA	101
		Actin_QR	GGATTCCAGCTGCTTCCATTC	101
Ht1-SST	AJ009757	HtSST_F	CACCACATGATGGCTTCATCCACC	1893
		HtSST_R	TAACCATCAAAGTTCGAAAGTCC	1893
		HtSST_QF	GACCGTCGTTTTGGACCTC	294
		HtSST_QR	GCTAGAACCACCACACCAAACGGTC	294
Ht1-FFT	AJ009756	HtFFT_F	GTCAGTCACCATGCAAACCC	1880
		HtFFT_R	AAAGGATAGCGATAGCCGGTA	1880
		HtFFT_QF	ATATCGAGGGCGTCTTATCCGGGTCTA	788
		HtFFT_QR	TCTCAAACTCTCGACTTCCTCAACGGG	788

Ht1-SST, Helianthus tuberosus sucrose:sucrose 1-fructosyltransferase; Ht1-FFT, H. tuberosus fructan:fructan 1-fructosyltransferase