Expression of Jerusalem artichoke (Helianthus tuberosus L.) fructosyltransferases, and high fructan accumulation in potato tubers

Applied Biological Chemistry

Table 2 Carbohydrate content of Jerusalem artichoke (Helianthus tuberosus L.) callus and transgenic potato tubers expressing Ht1-SST and/or Ht1-FFT

Plant material^a	Gene target	Sucrose (mg/g)	Inulin-type fructans
Plant material^a	Gene target	Sucrose (mg/g)	1-kestose (mg/g)	DP = 4 (mg/g)	DP = 5 (mg/g)	DP > 5 (mg/g)
Wild-type potato tuber	N.A.	5.39 ± 0.37	n.d.	n.d.	n.d.	n.d
Jerusalem artichoke callus	1-SST/1-FFT	6.58 ± 0.42	2.34 ± 0.82	1.32 ± 0.28	1.14 ± 0.29	6.07 ± 2.05
S4 tuber	1-SST	1.57 ± 0.48	3.38 ± 0.50	0.29 ± 0.01	n.d.	n.d.
S5 tuber	1-SST	2.83 ± 0.13	3.34 ± 0.80	0.27 ± 0.02	n.d.	n.d.
F4 tuber	1-FFT	2.60 ± 0.10	n.d.	n.d.	n.d.	n.d.
F5 tuber	1-FFT	4.91 ± 0.09	n.d.	n.d.	n.d.	n.d.
SnF1 tuber	1-SST/1-FFT	4.75 ± 0.04	1.66 ± 0.04	0.88 ± 0.15	0.60 ± 0.15	1.11 ± 0.11

N.A. not applicable, n.d. not detectable, DP degree of polymerization
^aS4 and S5, independent transgenic potato lines expressing Ht1-SST; F4 and F5, independent transgenic potato lines expressing Ht1-FFT; SnF1, transgenic potato line co-expressing Ht1-SST and Ht1-FFT