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Applied Biological Chemistry
Fig. 9 | Applied Biological Chemistry

Fig. 9

From: Evidence of multi-functional peptide activity: potential role of KT2 and RT2 for anti-inflammatory, anti-oxidative stress, and anti-apoptosis properties

Fig. 9

Anti-apoptosis activity of RT2. Apoptotic cells of tested macrophages RAW 264.7 were assigned by Annexin V-FITC/propidium iodide (PI) staining and investigated by flow cytometry. Profiles of Annexin V-FITC/PI staining of untreated cells (a), inflamed cells (b), inflamed cells treated with 5 μg/ml of RT2 (c), inflamed cells treated with 15 of μg/ml RT2 (d), and inflamed cells treated with 30 μg/ml RT2 (e) are shown. Representative percentages of apoptotic cells, where the population of cells stained by Annexin V-FITC(+)/PI(−) and Annexin V-FITC(+)/PI(+) are indicated as early and late apoptosis, respectively. Total apoptosis is indicated as the sum of percentages of early and late apoptosis (f). Bars depict the mean and standard deviations. Different lowercase letters on the top of individual bars indicate statistically significant differences (p < 0.05)

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