Fig. 2

Effect of Hispidin on LPS induced TNF-α and IL-6 production in RAW264.7 macrophage cells. Cells were pre-treated with 20 µg/ml Hispidin for 30 min, followed by treatment with LPS (1 µg/ml) for the indicated durations. a, b IL-6 and c, d TNF-α productions were measured in the culture media by enzyme-linked immunosorbent assay (ELISA). Data are presented as the mean ± standard error of the mean for three different samples. *P < 0.05, and ***P < 0.001