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Applied Biological Chemistry
Fig. 4 | Applied Biological Chemistry

Fig. 4

From: Cudrania tricuspidata leaf extracts and its components, chlorogenic acid, kaempferol, and quercetin, increase claudin 1 expression in human keratinocytes, enhancing intercellular tight junction capacity

Fig. 4

The effect of three major components of WECT on tight junction. a Chemical structure of chlorogenic acid, kaempferol, and quercetin. b Immunoblot analysis showing increased claudin 1 protein by chlorogenic acid, kaempferol, and quercetin. Band intensity of claudin 1 was normalized by tubulin. c Increased claudin 1 distribution throughout the tight junction. WECT were treated for positive control. Cells were stained with anti-claudin 1 (green), anti-tubulin alpha (red), and DAPI for nuclei (blue). Scale bars indicate 10 μm. d Dispase assay showing that enhanced tight junction capacity by the components of WECT. Number of fragments were counted. ***p < 0.001 (two-sided t-test, versus control)

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