Simultaneous determination of the metabolites of the herbicide metazachlor in agricultural crops by LC–MS/MS

Applied Biological Chemistry

Table 2 Validation results for the determination of 479M04, 479M08, and 479M16 in agricultural samples

Compound	Sample	Recovery ± RSD^a(%)		Linearity (r²)	LOQ (mg/kg)
Compound	Sample	Lab1^b	Lab2^c	Linearity (r²)	LOQ (mg/kg)
479M04	Hulled rice	98.7 ± 2.9–119.4 ± 2.8	79.6 ± 6.9–106.5 ± 14.4	0.9992	0.01
	Potato	87.1 ± 3.9–92.4 ± 3.7	72.0 ± 4.0–110.2 ± 1.1	0.9984
	Soybean	85.9 ± 6.8–89.4 ± 9.5	95.6 ± 7.3–98.1 ± 3.6	0.9999
	Mandarin	88.8 ± 2.6–94.0 ± 0.6	78.8 ± 15.9–86.3 ± 2.5	0.9995
	Green pepper	89.7 ± 3.4–100.0 ± 7.0	82.9 ± 9.5–88.5 ± 7.1	0.9990
479M08	Hulled rice	90.8 ± 10.9–100.6 ± 8.6	82.2 ± 2.4–94.8 ± 0.8	0.9999	0.01
	Potato	81.1 ± 6.7–88.4 ± 5.1	72.6 ± 3.3–97.0 ± 10.1	0.9994
	Soybean	79.3 ± 12.1–83.3 ± 7.4	86.9 ± 8.8–93.3 ± 9.5	0.9999
	Mandarin	75.6 ± 4.6–88.8 ± 4.1	77.3 ± 8.0–88.8 ± 14.5	0.9997
	Green pepper	82.0 ± 5.2–93.2 ± 8.5	76.4 ± 11.9–95.9 ± 13.1	0.9995
479M16	Hulled rice	101.6 ± 1.8–116.9 ± 3.0	83.3 ± 2.5–87.3 ± 9.5	0.9993	0.01
	Potato	83.3 ± 6.6–94.1 ± 4.6	91.9 ± 3.4–101.5 ± 5.1	0.9993
	Soybean	84.5 ± 4.4–90.2 ± 10.3	76.4 ± 9.1–88.8 ± 9.6	0.9998
	Mandarin	77.4 ± 4.2–96.2 ± 2.5	84.0 ± 1.8–91.6 ± 4.4	0.9996
	Green pepper	85.7 ± 2.9–92.4 ± 6.0	72.4 ± 11.0–89.3 ± 4.3	0.9996

For each of the five samples, standards at fortification levels of 0.01, 0.1, and 0.5 mg/kg were used
^aMean recovery values for 5 (Lab1) or 3 (Lab2) repetitions with relative standard deviation
^bMinistry of Food and Drug Safety
^cChonnam National University