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Fig. 1 | Applied Biological Chemistry

Fig. 1

From: Costunolide inhibits inflammation in LPS-induced RAW264.7 cells and ameliorates gastric acid reflux-induced esophageal injury in rat model

Fig. 1

The chemical structure of Cos (a), and effect of Cos on NO production (b), cell viability (c), observation and quantitative result of cell morphology (d) in RAW 264.7 cells induced by LPS. Concentration of nitrite oxide and cytotoxicity were measured by Griess Reagent and Cell Viability, Proliferation & Cytotoxicity Assay Kit, respectively. Morphology of cells was captured by optical microscopy and quantitative result of cell morphology was analysis using Image J program. Scale bar was 200 μm. Data are expressed as mean ± standard deviation. All experiments were performed in three independent experiments. ###p < 0.001 vs. normal cells; ***p < 0.001 vs. LPS-stimulated cells

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