Characterization of a novel endolysin from bacteriophage infecting Vibrio parahaemolyticus, vB_VpaP_KF2

Applied Biological Chemistry

Table 1 Antimicrobial spectrum of KF2_Lys endolysin

Bacterial strains			Relative lytic activity^a
Gram positive	Bacillus cereus	ATCC 14,579	+
	Listeria monocytogenes	KCCM 40,307	‒
	Staphylococcus aureus	ATCC 25,923	‒
Gram negative	Escherichia coli K12	ER2738	+
	Escherichia coli O157:H7	NCCP 1109-047	‒
	Lactobacillus acidophilus	KCTC 3145	‒
	Lactobacillus delbrueckii subsp. bulgaricus	ATCC 7995	‒
	Lactobacillus delbrueckii subsp. bulgaricus	ATCC 11,842	‒
	Salmonella Enteritidis	ATCC 13,076	‒
	Salmonella Enteritidis	KCCM 12,021	+
	Salmonella Typhimurium	KCCM 11,862	‒
	Vibrio alginolyticus	Isolate	++++
	Vibrio cholera	Isolate	+++
	Vibrio fluvialis	Isolate	+
	Vibrio metschnikovii	Isolate	+++
	Vibrio mimicus	Isolate	++++
	Vibrio parahaemolyticus	ATCC 27,969	++
	Vibrio parahaemolyticus	ATCC 17,802	+
	Vibrio parahaemolyticus	Isolate KF1	+++
	Vibrio parahaemolyticus	Isolate KF2	+++
	Vibrio parahaemolyticus	Isolate KF3	++++
	Vibrio parahaemolyticus	Isolate KF4	++++
	Vibrio parahaemolyticus	Isolate KF5	++++
	Vibrio parahaemolyticus	Isolate KF6	+++
	Vibrio parahaemolyticus	Isolate KF7	+++
	Vibrio parahaemolyticus	Isolate KF8	+++
	Vibrio vulnificus	ATCC 27,562	++
	Vibrio vulnificus	Isolate	+

^aRelative lytic activity (%) = 100 − (OD₆₀₀ after 30 min * 100/OD₆₀₀ at 0 time)
0–10% −; 10–20% +; 20–30% ++; 30–40% +++; > 40% ++++