Fig. 4

Cell viability of Hela, AGS, HT29, Raw264.7 cells and cancer cells staining after treatment with different concentration of compounds for 24 h. Cell viability was measured by MTT assay a Hela Cell, b AGS Cell, c HT29, d Raw264.7. Effect of dibenzocyclooctadiene lignans from Schisandra chinensis fruit on the activation of apoptosis in HeLa (e), AGS (f), and HT-29 (g) cells. Hoechst staining was used to detect nuclear condensation of treated and untreated cells. Propidium iodide (PI) staining was used to measure the cell death caused by treatment with the compounds. The white arrows show the unstained cell, the yellow and green arrows show the necrotic and apoptotic cells. The percentage of apoptosis were showed in h, i and j