Glycolysis stimulation and storage protein accumulation are hallmarks of maize (Zea mays L.) grain filling

Kim, Jung-Tae; Yi, Gibum; Kim, Mi-Jung; Son, Beom-Young; Bae, Hwan-Hee; Go, Young Sam; Kim, Sun-Lim; Baek, Seong-Bum; Kim, Seung-Hyun; Chung, Ill-Min

doi:10.1186/s13765-020-00538-6

Applied Biological Chemistry

Table 1 Differentially accumulated proteins during grain filling in kernels of the purple waxy maize cultivar ‘Heukjinjuchal’

From: Glycolysis stimulation and storage protein accumulation are hallmarks of maize (Zea mays L.) grain filling

Spot no.	Protein	UniProt No.	Expect^a	GO^b	SC(%)^c	MW (kDa)^d	PI^e
1	Translationally controlled tumor protein	B6SIF5	3.30E − 17		67	18.7	4.53
2	Histone H2B	C4J4M8	0.27	Protein heterodimerization	54	16.1	10.02
3	Membrane steroid-binding protein 1	B4FPD1	0.014	Endomembrane system	45	11.0	5.35
13	Chaperonin	B4F848	1.80E − 05	Chaperone cofactor	60	25.7	8.49
14	Triosephosphate isomerase	B6T2R0	2.60E − 31	Glycolytic process	78	26.9	5.12
15	Probable 6-phospho-gluconolactonase	C0PF40	3.30E − 24	6-phospho-gluconolactonase activity	75	28.9	5.08
16	Actin-97	B4F989	8.20E − 26	ATP binding	55	41.9	5.24
17	Heat shock 70 kDa protein	B6U4A3	3.30E − 15	ATP binding protein folding	37	72.9	5.62
18	Phosphoglycerate mutase 1	B8A306	2.60E − 33	Glycolytic pathway	51	60.7	5.29
19	2,3-bisphosphoglycerate-independent phosphoglycerate mutase 1	C0HHU2	3.30E − 44	Manganese ion binding, glucose catabolic process	59	60.4	5.47
20	2,3-bisphosphoglycerate-independent phosphoglycerate mutase 1	C0HHU2	1.00E − 41	Manganese ion binding, glucose catabolic process	55	60.4	5.47
21	Globulin-2	Q7M1Z8	1.30E − 08	Storage protein	29	50.2	6.16
22	Globulin-2	Q7M1Z8	2.10E − 12	Storage protein	29	50.2	6.16
23	Globulin-2	Q7M1Z8	5.20E − 08	Storage protein	26	50.2	6.16
24	Globulin-2	Q7M1Z8	2.60E − 09	Storage protein	26	50.2	6.16
25	Globulin-2	Q7M1Z8	4.10E − 13	Storage protein	26	50.2	6.16
26	Globulin-2	Q7M1Z8	4.10E − 08	Storage protein	29	50.2	6.16
27	Globulin-2	Q7M1Z8	0.00034	Storage protein	27	50.2	6.16
28	17.4 kDa class I heat shock protein 3	B6TDB5	0.0059	Stress response	43	17.7	5.55
29	4-hydroxy-4-methyl-2-oxoglutarate aldolase	B6TN41	0.0023	Substrate for enzyme	42	18.3	5.78
30	17.5 kDa class II heat shock protein	B4F9K4	2.90E − 05	Stress response	27	17.9	5.95
37	Superoxide dismutase [Mn] 3.1, mitochondrial	P09233	1.60E − 07	Stress response	25	25.5	7.11
38	Lactoylglutathione lyase	C0PK05	1.30E − 12	Glyoxal pathway	55	32.4	5.82
39	General stress protein 39	B4FNZ9	1.00E − 36	Oxidoreductase activity	69	33.1	5.78
40	rRNA N-glycosidase	B6SK87	2.10E − 43	Defense response	55	33.4	5.83
42	Malate dehydrogenase, cytoplasmic	Q08062	3.30E − 35	Malate metabolic process	68	35.9	5.77
43	Sorbitol dehydrogenase	B6TEC1	2.60E − 25	Oxidoreductase activity, zinc ion binding	74	39.5	6.27
44	Legumin-like protein	Q84TL6	8.20E − 42	Storage protein	79	38.2	6.23
45	Ribosome inactivating protein 1	Q2XXF4	2.10E − 33	Defense response	63	33.1	6.02
46	Globulin-2	Q7M1Z8	2.60E − 33	Storage protein	54	50.2	6.16
47	Vicilin-like embryo storage protein	Q03865	1.00E − 20	Storage protein	44	66.6	6.23
48	Vicilin-like embryo storage protein	Q03865	2.10E − 18	Storage protein	41	66.6	6.23

^aExpect: Mascot expectation value
^bGO: Gene ontology
^cSC: Sequence coverage
^dMW: Theoretical molecular weight computed from amino acid sequence
^epI: Theoretical isoelectric point computed from amino acid sequence

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