Fig. 3

Synthesis of fraxetin from esculetin using two E. coli transformants. a Conversion of esculetin into scopoletin using E. coli harboring POMT7. E. coli harboring POMT7 was administered esculetin (S1), following which the reaction product was analyzed. P1 denotes the reaction product from scopoletin. b Synthesis of fraxetin from scopoletin using E. coli harboring AtS8H. The culture filtrate from E. coli harboring POMT7 was administered to the E. coli harboring AtS8H, and the reaction product was analyzed