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Applied Biological Chemistry
Fig. 3 | Applied Biological Chemistry

Fig. 3

From: Anti-inflammatory effects of 18-nor-ent-pimara-9(11),15-diene-4β-ol isolated from the roots of Aralia continentalis on LPS-induced in RAW264.7 cells

Fig. 3

The effect of 18-nor-ent-pimara-9(11),15-diene-4β-ol on NF-κB signaling activation in LPS-stimulated RAW264.7 cells. a, b RAW264.7 cells were pretreated with 18-nor-ent-pimara-9(11),15-diene-4β-ol for 6 h and then co-treated with LPS (1 μg/ml) for 20 min. c RAW264.7 cells were pretreated with 18-nor-ent-pimara-9(11),15-diene-4β-ol for 6 h and then co-treated with LPS (1 μg/ml) for 20 min. After the treatment, the cytosol and nucleus were prepared. For Western blot analysis, the cell lysates were subjected to SDS-PAGE and the Western blot was performed using antibodies against p-IκB-α, IκB-α and p65. β-actin was used as internal control

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