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Applied Biological Chemistry
Fig. 1 | Applied Biological Chemistry

Fig. 1

From: Ameliorative effects of ark clams (Scapharca subcrenata and Tegillarca granosa) on endothelial dysfunction induced by a high-fat diet

Fig. 1

Concentration–response curves of Ach-induced endothelium-dependent relaxation a and SNP-induced endothelium-independent relaxation b in the phenylephrine-contracted thoracic aorta of the control, HF, and AS groups. The western blot analysis of endothelial dysfunction-related proteins in thoracic aorta tissue and its relative intensities, p-Akt ser473 (c), p-eNOS-Ser1177 (d), and p-eNOS Thr495 (e). Immunohistochemistry staining of ET-1 in the thoracic aorta sample using antibody against ET-1 and visualized with peroxidase displaying different staining intensity (brown color) (f) and their immune positivity expressed as area % (g). Data are expressed as mean ± SEM (n = 8). #P < 0.05 vs. Control, *P < 0.05 vs. HF

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