Fig. 5

Effect of BPA on NF-κB activation and MAPK phosphorylation in RAW 264.7 macrophages. a Cells were activated with BPA (0.1–10 μg/mL) for 4 h. The phosphorylation levels of p65 were detected by western blot assay to analyze the translocation of NF-κB. b Macrophages were incubated with various concentrations of BPA for 4 h. Whole cell lysates were investigated by western blot assay using an anti-IκBα antibody. c RAW 264.7 macrophages were treated with indicated concentrations of BPA (0.1–10 μg/mL) for 20 min. The phosphorylation levels of MAPK were detected by western blot assay. d RAW 264.7 macrophages were incubated with the NF-κB inhibitor Bay 11-7082 for 2 h and then stimulated with BPA for 4 h. e RAW 264.7 macrophages were treated with BPA for 20 min in the absence or presence of the p38 MAPK inhibitor SB203580 (10 μM), the ERK1/2 inhibitor PD98059 (20 μM), and the JNK inhibitor SP600125 (10 μM). Whole cell lysates were analyzed by western blot assay. β-Actin protein levels were used as an internal control. Data are expressed as the mean ± S.E.M of three independent experiments. *p < 0.05, significantly different from the untreated group