Table 1 Primer sequences and real-time PCR conditions
From: Bisphenol A disrupts inflammatory responses via Nod-like receptor protein 3 pathway in macrophages
Gene | Forward primer (5′ → 3′) | Reverse primer (5′ → 3′) |
|---|---|---|
TNF-α | CCC TCA CAC TCA GAT CAT CTT CT | GCT ACG ACG TGG GCT ACA G |
IL-6 | CCA CGG CCT TCC CTA CTT C | TTG GGA GTG GTA TCC TCT GTG A |
IL-1β | TTG ACG GAC CCC AAA AGA TG | TGG ACA GCC CAG GTC AAA G |
IL-18 | CTG AAG AAA ATG GAG ACC TGG AA | TCC GTA TTA CTG CGG TTG TAC AGT |
GAPDH | TGC ATC CTG CAC CAC CAA | TCC ACG ATG CCA AAG TTG TC |