Fig. 2
From: β-Glucogallin isolated from Fusidium coccineum and its enhancement of skin barrier effects
The effects of FC and β-glucogallin on skin barrier development and hydration in HaCaT cells. The mRNA expression of FLG in FC and β-glucogallin treated HaCaT cells (a, c). The mRNA expression of HAS3 in FC and β-glucogallin treated HaCaT cells (b, d). HaCaT cells were pretreated with various concentrations of FC and β-glucogallin for 24 h. The mRNA expression of FLG and HAS3 was determined by RT-PCR and quantified by densitometric anlaysis. Results are expressed as mean ± SD (% control) of three independent experiments; *p < 0.05, **p < 0.01. FLG, Filaggrin; HAS3, Hyaluronan synthase 3; FC, Fusidium coccineum; CA, Centella asiatica