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Applied Biological Chemistry

Table 1 Calibration curves of flavonoids in lotus

From: Validation of an optimized HPLC/UV method for the quantification of flavonoids in lotus

Compound

tR

Calibration equation

Correlation factor, r2

RUT

Q3Glu

Q3Glc

K3Glc

I3Glc

MYR

QUE

8.1

8.9

9.2

12.7

13.7

15.6

25.3

Y = 917.46X + 3.4761

Y = 1158.4X + 28.395

Y = 1913.5X + 63.777

Y = 1267.8X + 10.943

Y = 865.57X + 4.8457

Y = 1058.7X−14.542

Y = 1849.5X−36.422

0.9999

0.9999

1.0000

0.9998

1.0000

0.9999

0.9997

  1. tR = retention times
  2. Y = peak area, X = concentration of standard (µg/mL)
  3. r2 = correlation coefficient for five data points in the calibration curve
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