Fig. 3
From: Double-stranded RNA confers resistance to pepper mottle virus in Nicotiana benthamiana
Effects of HC-pro dsRNA on suppression of virus replication and HC-pro mRNA cleavage pattern from dsRNA treatment targeting HC-pro transcript. In qRT-PCR results (a), bars indicate GFP expression level in inoculated leaves at 8 dpi. In the 5 dpi and 8 dpi (b), the level of virus replication was confirmed by observing GFP expression under UV light. At 12 dpi, the growth differences between plants resulting from virus infection are evident. RLM-5′ RACE PCR results on N. benthamiana leaves treated with dsRNA corresponding to the central region of the HC-Pro along with PepMoV inoculation. Each bar shows the arrangement of HC-Pro mRNA fragments in leaves applied with virus and dsRNA (c), predicted locations of small RNA pools generated from dsRNA treatment (d) and the virus only (e), respectively. The darkness of bar color indicates the number of repetitions of small RNAs