Fig. 1

Anthocyanin accumulation in Col-0 plant cultured on nutrient-deficient soil and short-day conditions. a The Arabidopsis leaf color changed in nutrient-deficient soils on the adaxial and abaxial sides. Scale bar indicates 1 cm in length. b Col-0 plants were grown for the indicated number of weeks under short-day conditions. Anthocyanin was quantified in different leaves from three to 6-week old plants. c More details of anthocyanin accumulations in the 1st and 2nd and 9th and 10th leaves were measured and compared. The 9th and 10th leaves at day 14 were omitted since they were not developed. * indicates a significant difference between leaves 1/2 and 9/10 (n ≥ 4, p < 0.01). d Transcript level comparisons of three MYB genes in juvenile and adult leaves under normal plant growth conditions. Plants were grown for 4 weeks under normal long-day growth condition. * indicates a significant difference between leaves 1/2 and 9/10 (n ≥ 3, p < 0.01). e–h The expression patterns of three MYB genes and primary TAS4 transcription in the 1st and 2nd and 9th and 10th leaves under nutrient-deficient conditions. Each leaf was taken at day 14, 21, 29, and 42 after planting. * indicates significant difference in comparison with the other developmental stage of leaves 1/2 or 9/10 (n ≥ 3, p < 0.01). RQ represents relative quantity of target genes