Responses of inflammation signaling pathway by saucerneol D from elicitor-treated Saururus chinensis on pro-inflammatory responses in LPS-stimulated Raw 264.7 cell

Applied Biological Chemistry

Table 1 TPC, saucerneol D content, HAase inhibition, and expression levels of pro-inflammatory factors in S. chinensis extract treated by elicitor

Experiment	Control	Group A	Group B
TPC (mg/g)	7.6 ± 0.7	10.3 ± 0.3	11.4 ± 0.5
Saucerneol D content (μg/g)	248.8 ± 5.4	377.0 ± 11.6	449.9 ± 17.4
HAase inhibition (%)	89.6 ± 1.2	93.9 ± 2.9	95.9 ± 0.8
NO production (%)	86.8 ± 1.8	75.3 ± 1.9	68.1 ± 3.1
iNOS expression (%)	70.3 ± 0.9	35.6 ± 2.1	39.1 ± 1.4
COX-2 expression (%)	90.2 ± 5.1	56.1 ± 3.2	53.3 ± 2.7

Control: no elicitor treatment, group A: 4.5 mg/mL of treatment, group B: 9.0 mg/mL. Raw 264.7 cell were treated with 25 μg/mL of extracts from S. chinensis (dissolved in DW) for 1 h prior to addition of LPS (1 μg/mL). The cells were further incubated with LPS for 24 h. Control cells were incubated with vehicle alone. The concentrations of nitrite and nitrate in the culture medium were monitored as described in the experimental procedures. Values are presented as the mean ± error (n = 6)