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Applied Biological Chemistry

Table 4 Ginsenoside content of BRG treated with mixed and sequential enzymes

From: Optimal bioconversion for compound K production from red ginseng root (C.A. Mayer) by sequential enzymatic hydrolysis and its characteristics

Enzyme treatment

Ginsenoside (mg/g)

1st (24 h)

2nd (48 h)

Rb1

Rd

F2

CK

Control

20.88 ± 0.01a*

4.60 ± 0.02a

2.01 ± 0.01f

–

Pyr + Rap1

–

–

0.17 ± 0.00e

18.65 ± 0.01a

3.32 ± 0.12d

Pyr-flo2

MFC

–

0.30 ± 0.005b

10.17 ± 0.018d

13.93 ± 0.041a

Pyr + Rap3

MFC

–

0.23 ± 0.001d

7.99 ± 0.011e

14.32 ± 0.028a

Pyr-flo4

Cel

–

0.26 ± 0.002c

17.47 ± 0.019b

4.13 ± 0.139b

Pyr + Rap5

Cel

–

0.18 ± 0.010e

15.81 ± 0.093c

3.70 ± 0.065c

  1. BRG; freeze-dried red ginseng extract treated with mixed and sequential enzymes
  2. Pyr; Pyr-flo, Rap; Rapidase C80MAX, MFC; Ultimase MFC, Cel; Celluclast 1.5 L
  3. 1Single-step treatment with 5% Pyr-flo and 5% Rapidase for 24 h
  4. 2Sequential-step treatment with 5% Pyr-flo for 24 h, followed by 5% Ultimase MFC for 48 h
  5. 3Sequential-step treatment with 5% Pyr-flo and 5% Rapidase for 24 h, by 5% Ultimase MFC for 48 h
  6. 4Sequential-step treatment with 5% Pyr-flo for 24 h, by 5% Celluclast 1.5 L for 48 h
  7. 5Sequential-step treatment with 5% Pyr-flo and 5% Rapidase for 24 h, by Celluclast 1.5 L for 48 h
  8. Data are expressed as mean ± SD
  9. * Different superscript letters in the same column mean significantly different at p < 0.05 by Duncan’s multiple range test
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