A rapid, simultaneous and quantitative analysis of 26 ginsenosides in white and red Panax ginseng using LC–MS/MS

Applied Biological Chemistry

Table 1 Optimized MRM transitions of ginsenosides in UHPLC-MS/MS analysis

No.	Ginsenoside	Monoisotopic mass	Adduct formula	MRM transitions
No.	Ginsenoside	Monoisotopic mass	Adduct formula	Precursor	Quantifier	Qualifier
1	Re	946.55	[M + Na]⁺	969.40	789.55	203.05
2	Rg1	800.49	[M + Na]⁺	823.50	643.30	203.05
3	Rh1(S)	638.44	[M + Na]⁺	661.30	203.10	22.95
4	F3	770.48	[M + Na]⁺	793.30	335.10	–
5	Rh1(R)	638.44	[M + Na]⁺	661.30	22.90	203.05
6	F1	638.44	[M + Na]⁺	661.20	203.10	481.30
7	Rb1	1108.60	[M + Na]⁺	1131.50	365.00	789.35
8	Rc	1078.59	[M + Na]⁺	1101.50	335.00	789.35
9	Rb2	1078.59	[M + Na]⁺	1101.50	789.35	335.05
10	PPT(S)	476.39	[M-2H₂O + H]⁺	441.30	43.35	123.05
11	Rd	946.55	[M + Na]⁺	969.40	789.35	203.05
12	XVII	946.55	[M + Na]⁺	969.40	365.10	–
13	Rg3(S)	784.50	[M-2H₂O + H]⁺	749.50	407.35	163.10
14	Rg3(R)	784.50	[M-2H₂O + H]⁺	749.50	163.10	407.35
15	F2	784.50	[M + Na]⁺	807.50	627.30	203.10
16	Mc	754.49	[M + Na]⁺	777.60	335.10	336.10
17	K	622.44	[M + Na]⁺	645.20	203.00	465.30
18	Rh2(S)	622.44	[M-2H₂O + H]⁺	587.40	407.30	123.10
19	Rh2(R)	622.44	[M-2H₂O + H]⁺	587.40	407.35	109.10
20	Rf	800.49	[M-H]⁻	799.50	475.45	100.90
21	Rg2(S)	784.50	[M-H]⁻	783.50	475.35	161.15
22	Rg2(R)	784.50	[M-H]⁻	783.50	475.45	119.10
23	Rg6	766.49	[M-H]⁻	765.50	101.10	161.10
24	F4	766.49	[M-H]⁻	765.50	101.10	161.10
25	Y	754.49	[M-H]⁻	753.50	149.15	89.05
26	Rg5	766.49	[M-H]⁻	765.50	101.05	161.20