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Applied Biological Chemistry
Fig. 3 | Applied Biological Chemistry

Fig. 3

From: Characterization of tea leaf metabolites dependent on tea (Camellia sinensis) plant age through 1H NMR-based metabolomics

Fig. 3

The OPLS-DA score (a and c) and loading (b and d) plots derived from 1H NMR spectra of tea leaf extracts, providing a pairwise plot for metabolic comparison between tea plants aged 8 (8 y) and 25 (25 y) years old collected in April 2016 (a and b) and in May 2015 (c and d). In the loading plot (b and d), the upper section represents the higher levels of tea leaf metabolites in old tea plants compared to young tea plants, whereas the lower section denotes metabolites lower in old tea plants. The color code in the loading plot corresponds to the correlation between the variables. All OPLS-DA modes were generated with one predictive component and one orthogonal component and were validated by a permutation test (Additional file 1: Fig. S2). Ala, alanine; Ara, 2-O-(β-l-arabinopyranosyl)-myo-inositol; Asp, aspartic acid; EC, epicatechin; EGC, epigallocatechin; FA, fatty acids; GABA, γ-aminobutyric acid; GC, gallocatechin; Gln, glutamine; Val, valine. Asterisks represent epigallocatechin 3-O-(3″-O-methyl) gallate (EGCG3ʺMe)

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