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Applied Biological Chemistry
Fig. 2 | Applied Biological Chemistry

Fig. 2

From: Potato extract inhibits lipase activity and ameliorates gut microbiome dysbiosis and weight gain in mice fed a high-fat diet

Fig. 2

a Kinetics of PI during lipase inhibition based on Lineweaver–Burk double reciprocal plotting when 4-MUO was used as a substrate. The x-intercept (Km) is unaffected while different concentrations have slopes, and the y-intercepts (varied Vmax) show non-competitive inhibition. Comparison of the inhibitory affinity of PI and orlistat on the hydrolysis of 4-MUO by pancreatic lipase. The equations of the dose–response curves were obtained from the least-squares regression of the %-inhibition (response) at different logarithmic inhibitor concentrations (doses) of PI (b) and orlistat (c) to calculate the half maximal inhibitory concentration (IC50). The values given are the means of the duplicate

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