Potato extract inhibits lipase activity and ameliorates gut microbiome dysbiosis and weight gain in mice fed a high-fat diet

Applied Biological Chemistry

Table 1 Lipase inhibitory activities in the extracts of different solvent phases

Extraction	Volume of reconstituted extract (mL)	Specific inhibitory activity^a	Relative inhibitory activity (%)^b
Dichloromethane	15	n.d.^c	0
Ethyl acetate	15	0.58 ± 0.017	24.4 ± 1.28
Butanol	15	0.45 ± 0.018	18.9 ± 1.00
Water	35	0.58 ± 0.001	56.7 ± 2.46

^aThe specific lipase inhibition was calculated by dividing the difference between the total and the residual lipase activity with the total lipase activity (negative control) for 10 µL of the sample. 4-Methylumbelliferyl oleate (4-MUO) was used as a substrate. Orlistat (positive control) revealed 100% inhibition at a concentration of 0.01 mg/mL
^bThe relative inhibitory activity was calculated as a fraction of the inhibitory activity from the total activity in all different solvent phases
^cNot detected