Potato extract inhibits lipase activity and ameliorates gut microbiome dysbiosis and weight gain in mice fed a high-fat diet

Applied Biological Chemistry

Table 2 Lipase inhibitory activities of the C18 column fractions

Fraction	Volume of reconstituted extract (mL)	Specific inhibitory activity^a	Yield (%)^b
Concentrated extract	100	0.50	100
C18 flow-through	50	0.88	88
C18 wash	50	0.70	70

^aThe specific lipase inhibition was calculated by dividing the difference between the total and the residual lipase activity with the total lipase activity (negative control) for 10 µL of the sample. 4-MUO was used as a fluorescent substrate. Orlistat revealed 100% inhibition (positive control) at a concentration of 0.01 mg/mL
^bThe yield was calculated as a relative inhibitory activity in each fraction compared by the total activity in the “concentrated extract”