Fig. 4

Apoptosis of neuronal cells grown on the CM-PAF. CM-PAF was prepared from the cultured media where LPS-treated BV-2s were grown after treating various concentration of PAF. Control means the cultured media where non-treated BV-2s were grown. Apoptosis and necrosis of N2as were analyzed in the N2as grown on a control, b CM-PAF (0 μg/mL), c CM-PAF (10 μg/mL), d CM-PAF (50 μg/mL), e CM-PAF (100 μg/mL). Expression of Bcl-2 and Bax in neuronal cells grown on CM-PAFs. CM-PAF was prepared from the cultured media where LPS-treated BV-2s were grown after treating various concentration of PAFE. Control means the cultured media where non-treated BV-2s were grown. N2as were grown on control or CM-PAFs and then f expression of Bcl-2, Bax, and β-actin in those cells were shown by western blot. g The Bax/Bcl-2 ratio was calculated based on the relative density shown in the western blot by using the ImageJ software