Table 1 Plasmids, bacterial strains, and primers used in this study
From: Biosynthesis of resveratrol using metabolically engineered Escherichia coli
Plasmids/Strains | Description | Sources or reference |
|---|---|---|
Plasmids | ||
pACYCDuet | P15A ori, Cmr | Novagen |
pCDFDuet | CloDE13 ori, Strr | Novagen |
PA-SeTAL | pACYCDuet carrying TAL from Saccharothrix espanaensis | |
PC-Pc4CL-VvSTS(P) | pCDFDuet carrying Pc4CL from Petroselinum crispum and VvSTS from Vitis vinifera. The genes are regulated by the respective T7 promoter | This study |
PC-Sc4CL-VvSTS(P) | pCDFDuet carrying Sc4CL from Streptomyces coelicolar A(3) and VvSTS from Vitis vinifera. The genes are regulated by the respective T7 promoter | This study |
PC-Os4CL-VvSTS(P) | pCDFDuet carrying Os4CL from Oryza sativa and VvSTS from Vitis vinifera. The genes are regulated by the respective T7 promoter | This study |
PC-At4CL-VvSTS(P) | pCDFDuet carrying At4CL from Arabidopsis thaliana and VvSTS from Vitis vinifera. The genes are regulated by the respective T7 promoter | This study |
PC-Le4CL-VvSTS(P) | pCDFDuet carrying Le4CL from Lithospermum erythrorhizon and VvSTS from Vitis vinifera. The genes are regulated by the respective T7 promoter | This study |
PC-Os4CL-AhSTS(P) | pCDFDuet carrying Os4CL from Oryza sativa and AhSTS from Arachis hypogaea. The genes are regulated by the respective T7 promoter | This study |
PC-Os4CL-PaSTS(P) | pCDFDuet carrying Os4CL from Oryza sativa and PaSTS from Picea abies. The genes are regulated by the respective T7 promoter | This study |
PC-AhSTS-Os4CL(O) | pCDFDuet carrying Os4CL from Oryza sativa and AhSTS from Arachis hypogaea. The genes are regulated by one T7 promoter | This study |
PC-PaSTS-Os4CL(O) | pCDFDuet carrying Os4CL from Oryza sativa and PaSTS from Picea abies. The genes are regulated by one T7 promoter | This study |
PC-VvSTS-Os4CL(O) | pCDFDuet carrying Os4CL from Oryza sativa and VvSTS from Vitis vinifera. The genes are regulated by one T7 promoter | This study |
PA-aroG-SeTAL-tyrA | pACYCDuet carrying TAL from S. espanaensis, aroG, and tyrA from E. coli | Kim el al. [18] |
PA-aroGfbr-ppsA-tktA-SeTAL-tyrAfbr | pACYCDuet carrying TAL from S. espanaensis, aroGfbr, ppsA, tktA, and tyrAfbr from E. coli | Kim el al. [18] |
Strains | ||
BL21(DE3) | F− ompT hsdSB(rB− mB−) gal dcm lon (DE3) | |
B-TP | BL21(DE3) ΔtyrR::FRT-ΔpheA::FRT-kanR-FRT | Kim et al. [18] |
B-TPFI | BL21(DE3) ΔtyrR::FRT-ΔpheA::FRT-ΔfumC::FRT-ΔicdA::FRT-kanR-FRT | This study |
BP-Pc4CL | BL21(DE3) harboring PC-Pc4CL-VvSTS(P) | This study |
BP-Os4CL | BL21(DE3) harboring PC-Os4CL-VvSTS(P) | This study |
BP-At4CL | BL21(DE3) harboring PC-At4CL-VvSTS(P) | This study |
BP-Sa4CL | BL21(DE3) harboring PC-Sa4CL-VvSTS(P) | This study |
BP-Le4CL | BL21(DE3) harboring PC-Le4CL-VvSTS(P) | This study |
BP-AhSTS | BL21(DE3) harboring PC-Os4CL-AhSTS(P) | This study |
BP-PaSTS | BL21(DE3) harboring PC-Os4CL-PaSTS(P) | This study |
BO-VvSTS | BL21(DE3) harboring PC-Os4CL-VvSTS(O) | This study |
BO-AhSTS | BL21(DE3) harboring PC-Os4CL-AhSTS(O) | This study |
BO-PaSTS | BL21(DE3) harboring PC-Os4CL-PaSTS(O) | This study |
BL101 | BL21(DE3) harboring PA-SeTAL and PC-VvSTS-Os4CL(O) | This study |
BL102 | BL21(DE3) harboring PA-aroG-SeTAL-tyrA and PC-VvSTS-Os4CL(O) | This study |
BL103 | BL21(DE3) harboring PA-aroGfbr-ppsA-tktA-SeTAL-tyrAfbr and PC-VvSTS-Os4CL(O) | This study |
BTP-S | BTP harboring PA-aroGfbr-ppsA-tktA-SeTAL-tyrAfbr and PC-VvSTS-Os4CL(O) | This study |
BTPFI-S | BTPFI harboring PA-aroGfbr-ppsA-tktA-SeTAL-tyrAfbr and PC-VvSTS-Os4CL(O) | This study |
Primers | ||
tyrR-F-Del | GTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGaattaaccctcactaaagggcg | |
tyrR-R-Del | TTGCACCATCAGGCATATTCGCGCTTACTCTTCGTTCTTCTTCTGACTCAtaatacgactcactatagggctc | |
pheA-F-Del | CCTCCCAAATCGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGaattaaccctcactaaagggcg | |
pheA-F-Del | CACATCATCCGGCACCTTTTCATCAGGTTGGATCAACAGGCACTACGTTCtaatacgactcactatagggctc | |
fumC-F-del | TTAACGCCCGGCTTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCaattaaccctcactaaagggcg | |
fumC-R-del | ATGAATACAGTACGCAGCGAAAAAGATTCGATGGGGGCGATTGATGTCCCtaatacgactcactatagggctc | |
icdA-F-del | ATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAaattaaccctcactaaagggcg | |
icdA-R-del | TTACATGTTCTTGATGATCGCATCACCAAATTCTGAACATTTCAGCAGTTtaatacgactcactatagggctc | |
Os4CL-BamHI | ATggatccGATGGGGTCGGTGGCGGCGG | |
Os4CL-NotI | ATgcggccgcTTAGCTGCTTTTGGGCGC | |
Os4CL2-HindIII-RBS | ATaagcttAGGAGGATTACAAAATGATCACGGTGGCG | |
Os4CL2-NotI | ATgcggccgcTCAGCACGCCGCGAGCTTGGCT | |
Sc4CL-BamHI | AAggatccGATGTTCCGCAGCGAGTACGCA | |
Sc4CL-NotI | AAgcggccgcTCATCGCGGCTCCCTGAGCT | |
Le4CL-BamHI | ATggatccGATGGACACTCAAACCAAAA | |
Le4CL-NotI | CAgcggccgcTAATTGTGTACACCATTTG | |
Pc4CL-EcoRI | ATgaattcATGGGAGACTGTGTAGCACC | |
Pc4CL-NotI | ATgcggccgcTTATTTGGGAAGATCACCGG | |
VvSTS-EcoRI | ATgaattcGATGGCTTCAGTCGAGGAATTT | |
VvSTS-NotI | ATgcggccgcTTAATTTGTAACCATAGGAA | |
VvSTS-HindIII | GCaagcttTTAATTTGTAACCATAGGAA | |
VvSTS-smaI | ATcccgggGATGGCTTCAGTCGAGGAATTT | |
VvSTS-XhoI | ATctcgagTTAATTTGTAACCATAGGAATG | |
AhSTS-EcoRV | ATgatatcGATGGTGTCTGTGAGTGGAATT | |
AhSTS-XhoI | CAtctcgaGTTATATGGCCACACTGCGGAG | |
AhSTS-EcoRV | ATgatatcGATGGTGTCTGTGAGTGGAATT | |
AhSTS-XhoI | CATctcgagTTATATGGCCACACTGCGGAG | |
AhSTS-EcoRI | ATgaattcGATGGTGTCTGTGAGTGGAATCCGCA | |
AhSTS-HindIII | ATaagcttTTATATGGCCACACTGCGGAGA | |
AhSTS-XbaI | ATtctagaTTATATGGCCACACTGCGGAGAAC | |
AhSTS-HindIII silencing-F | TGACGCGCTCAATAAgGCTTTTGATCCATTG | |
AhSTS-HindIII silencing-R | CAATGGATCAAAAGCCTTATTGAGCGCGTCA | |
PsSTS-EcoRV | ATgatatcGATGTCTGCAGGAATGACTGTT | |
PaSTS-SalI | ATgtcgacTCATGGAAGGAGAACGCTCTTA | |
PsSTS-EcoRI | ATgaattcGATGTCTGTAGGAATGGGCGTTGATTTGGAGGCTTTCAGGAA | |
PsSTS-HindIII | ATaagcttTCATGGAAGGAGAACGCTCTTA | |
SeTAL-aflII | atcttaagTCATCCGAAATCCTTCCCGTC | |
T7-SeTAL-NotI | ATgcggccgcGGATCTCGACGCTCTCCCTTAT | |
aroG-EcoRI | ATgaattcgATGAATTATCAGAACGACGAT | |
aroG-SalI | ATgtcgacTTACCCGCGACGCGCTTTTACT | |
tyrA-NdeI | ATcatatgATGGTTGCTGAATTGACCGCAT | |
tyrA-KpnI | CATggtaccTTACTGGCGGTTGTCATTCGC | |
ppsA-RBS-XhoI | ATctcgagAGGAGGCCATCCATGTCCAACAATGGCTCGTC | |
ppsA-SalI-NotI | ATgcggccgcGCTgtcgacTTATTTCTTCAGTTCAGCCAG | |
tktA-RBS-XhoI | ATctcgagAGGAGGCCATCCATGTCCTCACGTAAAGAGCT | |
tktA-NotI | CATgcggccgcTTACAGCAGTTCTTTTGCTTTC | |