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Applied Biological Chemistry
Fig. 3 | Applied Biological Chemistry

Fig. 3

From: Murrayanine suppresses the proliferation and metastasis of human breast cancer cells via induction of apoptosis and inhibition of RANK/RANKL signaling pathway

Fig. 3

Induction of apoptosis in breast cancer cells by murrayanine via RANK/RANKL signaling pathway. a Viability of untreated control and murrayanine treated (18 µM) CAMA-1 cancer cells at 0, 12, 24, 36 or 48 h. b Viability of si-NC RNA and si-RANK transfected CAMA-1 cancer cells at 0, 12, 24, 36 or 48 h as determined by MTT assay. c EdU staining analysis showing proliferation of untreated, murrayanine treated (18 µM) si-NC RNA, and si-RANK transfected CAMA-1 cancer cells at 24 h culture duration. d Relative percentage of EdU positive in murrayanine treated (18 µM) si-NC RNA, and si-RANK transfected CAMA-1 e Annexin V/PI assay showing percent apoptosis of murrayanine treated (18 µM) si-NC RNA, and si-RANK transfected CAMA-1 cells f Western blots showing the expression of Caspase-3, Bcl-xl, Bcl-2 and Bax in murrayanine treated (18 µM) si-NC RNA, and si-RANK transfected CAMA-1 cells. Experiments were performed using three replicates and values are presented as mean ± SD (*P < 0.05)

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