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Applied Biological Chemistry
Fig. 4 | Applied Biological Chemistry

Fig. 4

From: Murrayanine suppresses the proliferation and metastasis of human breast cancer cells via induction of apoptosis and inhibition of RANK/RANKL signaling pathway

Fig. 4

Inhibition of breast cancer cell migration and invasion by murrayanine and RANK silencing. a Wound healing assay showing migration of untreated, murrayanine treated (18 µM), si-NC RNA, and si-RANK transfected CAMA-1 cells. b Transwell assay showing invasion of untreated, murrayanine treated (18 µM), si-NC RNA, and si-RANK transfected CAMA-1. c Western blots showing the expression of MMP-2 and MMP-9 in untreated, murrayanine treated (18 µM), si-NC RNA, and si-RANK transfected CAMA-1. d Western blotting of E-cadherin (epithelial marker), vimentin and N-cadherin (both mesenchymal marker) proteins from untreated, murrayanine treated (18 µM), si-NC RNA, and si-RANK transfected CAMA-1 cells. e Relative expression analysis of E-cadherin protein from untreated, murrayanine treated (18 µM), si-NC RNA, and si-RANK transfected CAMA-1 cells. g Relative expression analysis of N-cadherin protein from untreated, murrayanine treated (18 µM), si-NC RNA, and si-RANK transfected CAMA-1 cells. Experiments were performed using three replicates and values are presented as mean ± SD (*P < 0.05)

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