Fig. 3

Quantitative real-time PCR analysis to determine the quantity of yeast genomic DNA content relative to a universal gene after 24 h of incubation for analysis of DNA proportions in manure. The strains were incubated under aerobic and anaerobic conditions at 30 °C. Values are the mean ± SEM. Error bars represent the standard error (n = 3). a DNA proportion of Saccharomyces boulardii CNCM I-1079 (SB), b DNA proportion of Saccharomyces cerevisiae KCTC 7107 (SC). *p < 0.05, ***p < 0.001, compared between 0 and 24 h DNA proportion in the same group and same condition, indicates statistical significance (0 vs. 24 h of SB in the aerobic condition: f-value = 9.00, degree of freedom (DF) = 4, t-value = − 2.95, p = 0.0419; 0 vs. 24 h of SB in the anaerobic condition: f-value = 2.98, DF = 4, t-value = − 2.81, p = 0.0481; 0 vs. 24 h of SC in the aerobic condition: f-value = 2.22, DF = 4, t-value = − 13.28, p = 0.0002; 0 vs. 24 h of SC in the anaerobic condition: f-value = 44.65, DF = 2.0895, t-value = − 5.81, p = 0.0256)