Characterisitcs of Saccharomyces boulardii for reducing ammonia emission from livestock manure

Applied Biological Chemistry

Table 1 List of PCR primer pairs used for qPCR

Target strain	Primer name	Sequence(5′–3′)	Product size (bp)	References
Universal yeast	YEASTF	GAGTCGAGTTGTTTGGGAATGC	124	[24]
Universal yeast	YEASTR	TCTCTTTCCAAAGTTCTTTTCATCTTT	124	[24]
Saccharomyces cerevisiae	SCDF	AGGAGTGCGGTTCTTTG	310	[25]
Saccharomyces cerevisiae	SCDR	TACTTACCGAGGCAAGCTACA	310	[25]
Total bacteria	TBF	CGGTGAATACGTTCCCGG	142	[26]
Total bacteria	TBR	TACGGCTACCTTGTTACGACTT	142	[26]
Proteus mirabilis	ureRF	GGTGAGATTTGTATTAATGG	225	[27]
Proteus mirabilis	ureRR	ATAATCTGGAAGATGACGAG	225	[27]