From: Biosynthesis of resveratrol derivatives and evaluation of their anti-inflammatory activity
Plasmids and E. coli strains | Relevant properties and genetic markers | Sources and references |
---|---|---|
Plasmids | ||
 pETDuet | F1 ori, Ampr | Novagen |
 pCDFDuet | CDF ori, Smr | Novagen |
 pACYCDuet | P15A ori, Cmr | Novagen |
 pGEX 5X-1 | pBR322 ori, Ampr | GE Healthcare |
 pC-SeTAL-VvSTS-Os4CL | pCDFDuet carrying TAL from Saccharothrix espanaensis, STS from Vitis vinifera and 4CL from Oryza sativa | This study |
 pG-ROMT9 | pGEX 5X-1 carrying ROMT9 from Oryza sativa | [40] |
 pG-SOMT2 | pGEX 5X-1 carrying SOMT2 from Glycine max | [41] |
 pG-HpaBC | pGEX 5X-1 carrying HpaBC from E. coli | [39] |
 pE-SOMT2-HpaBC | pGEX 5X-1 carrying SOMT2 from Glycine max and HpaBC from E. coli | This study |
 pE-ROMT15-HpaBC | pETDuet carrying ROMT15 from Oryza sativa and HpaBC from E. coli | This study |
 pA-aroG-tyrA | pACYCDuet carrying aroG and tyrA from E. coli | This study |
 pA-aroGf-tyrAf | pACYCDuet carrying aroGf and tyrAf from E. coli | This study |
 pA-aroGf-tyrAf-ppsA-tktA | pACYCDuet carrying aroGf, tyrAf, ppsA and tktA from E. coli | This study |
 pA-aroL-aroGf-tyrAf-ppsA-tktA | pACYCDuet carrying aroL, aroGf, tyrAf, ppsA and tktA from E. coli | This study |
 pA-aroC-aroA-aroL-ydiB-aroD-aroB-aroGf-tyrAf-ppsA-tktA | pACYCDuet carrying aroC, aroA, aroL, ydiB, aroD, aroB, aroGf, tyrAf, ppsA and tktA from E. coli | This study |
 pA-aroC-aroA-aroL-aroE-aroD-aroB-aroGf-tyrAf-ppsA-tktA | pACYCDuet carrying aroC, aroA, aroL, aroE, aroD, aroB, aroGf, tyrAf, ppsA and tktA from E. coli | This study |
Strains | ||
 BL21 (DE3) | F− ompT hsdSB(\({r}_{B}^{-}{m}_{B}^{-}\)) gal dcm lon (DE3) | Novagen |
 B-RS1 | BL21 harboring pC-SeTAL-VvSTS-Os4CL and pACYCDuet | This study |
 B-RS2 | BL21 harboring pC-SeTAL-VvSTS-Os4CL and pA-aroG-tyrA | This study |
 B-RS3 | BL21 harboring pC-SeTAL-VvSTS-Os4CL and pA-aroGf-tyrAf | This study |
 B-RS4 | BL21 harboring pC-SeTAL-VvSTS-Os4CL and pA-aroGf-tyrAf-ppsA-tktA | This study |
 B-RS5 | BL21 harboring pC-SeTAL-VvSTS-Os4CL and pA-aroL-aroGf-tyrAf-ppsA-tktA | This study |
 B-RS6 | BL21 harboring pC-SeTAL-VvSTS-Os4CL and pA-aroC-aroA-aroL-ydiB-aroD-aroB-aroGf-tyrAf-ppsA-tktA | This study |
 B-RS7 | BL21 harboring pC-SeTAL-VvSTS-Os4CL and pA-aroC-aroA-aroL-aroE-aroD-aroB-aroGf-tyrAf-ppsA-tktA | This study |