Table 2 Validation results for the analytical method of 64 compounds in 3 kinds of food matrices
Compounds | Spiking level (µg/kg) | Flatfish (n = 5) | Eel (n = 5) | Shrimp (n = 5) | |||
|---|---|---|---|---|---|---|---|
Rec. (%) | CV (%) | Rec. (%) | CV (%) | Rec. (%) | CV (%) | ||
Acetanilide | 5 | 117.4 | 10.6 | 73.7 | 19.0 | 107.1 | 10.9 |
10 | 93.4 | 12.8 | 80.5 | 6.9 | 98.5 | 14.4 | |
20 | 75.0 | 6.2 | 72.8 | 4.1 | 90.5 | 13.9 | |
Antipyrine | 5 | 88.0 | 11.6 | 67.4 | 24.8 | 100.5 | 13.0 |
10 | 79.0 | 2.2 | 76.7 | 4.1 | 100.6 | 8.6 | |
20 | 71.3 | 6.2 | 74.5 | 9.1 | 95.0 | 11.4 | |
Arprinocid | 5 | 112.1 | 3.4 | 67.2 | 1.4 | 100.3 | 5.6 |
10 | 85.8 | 7.1 | 80.7 | 5.4 | 93.8 | 9.7 | |
20 | 70.9 | 4.9 | 76.5 | 3.5 | 89.0 | 7.1 | |
Azaperone | 5 | 113.9 | 9.9 | 98.4 | 7.7 | 88.0 | 9.8 |
10 | 98.0 | 11.6 | 87.1 | 11.2 | 101.8 | 29.6 | |
20 | 70.0 | 10.8 | 77.6 | 8.7 | 84.1 | 18.0 | |
Azaperol | 5 | 103.0 | 6.4 | 60.6 | 12.6 | 92.1 | 6.5 |
10 | 91.2 | 7.9 | 79.2 | 10.1 | 97.5 | 11.8 | |
20 | 73.8 | 4.9 | 84.2 | 6.8 | 89.1 | 7.5 | |
Bactitracin | 5 | 93.4 | 13.3 | 82.6 | 24.6 | 115.8 | 11.4 |
10 | 65.3 | 29.3 | 74.7 | 3.5 | 89.8 | 12.7 | |
20 | 78.4 | 8.8 | 81.3 | 10.6 | 78.9 | 6.8 | |
Berberine | 5 | 116.4 | 14.0 | 65.4 | 7.5 | 97.6 | 13.1 |
10 | 88.5 | 4.8 | 78.1 | 4.0 | 98.4 | 14.9 | |
20 | 71.6 | 8.0 | 74.1 | 2.0 | 90.0 | 13.0 | |
Caffeine | 5 | 89.7 | 14.2 | 74.7 | 18.7 | 115.3 | 8.4 |
10 | 73.9 | 10.5 | 77.9 | 14.0 | 98.6 | 5.3 | |
20 | 68.4 | 7.3 | 70.6 | 11.1 | 97.2 | 5.5 | |
Carazolol | 5 | 97.5 | 12.8 | 68.7 | 9.1 | 95.5 | 8.4 |
10 | 84.2 | 9.1 | 78.2 | 5.6 | 87.2 | 11.8 | |
20 | 70.5 | 6.0 | 74.7 | 2.6 | 84.5 | 5.2 | |
Carbendazim | 5 | 120.4 | 3.9 | 104.4 | 6.4 | 108.8 | 9.4 |
10 | 88.9 | 5.9 | 88.9 | 2.2 | 93.2 | 8.2 | |
20 | 68.1 | 9.1 | 65.4 | 9.9 | 83.3 | 7.3 | |
Cefazolin | 5 | 110.1 | 20.9 | 104.2 | 26.2 | 118.8 | 26.0 |
10 | 79.5 | 9.2 | 88.1 | 22.3 | 91.0 | 24.5 | |
20 | 70.7 | 9.9 | 72.3 | 13.9 | 92.7 | 22.0 | |
Cefoperazone | 5 | 79.3 | 13.1 | 62.2 | 26.9 | 110.0 | 21.2 |
10 | 81.1 | 17.2 | 84.2 | 22.0 | 96.6 | 18.3 | |
20 | 73.9 | 16.5 | 78.8 | 5.7 | 109.8 | 7.7 | |
Chlorpromazine | 1 | 118.0 | 11.4 | 119.5 | 6.1 | 81.6 | 24.4 |
2 | 86.0 | 9.4 | 80.5 | 17.1 | 114.5 | 14.9 | |
10 | 63.1 | 9.6 | 78.2 | 22.3 | 87.6 | 23.0 | |
Clenbuterol | 1 | 69.1 | 26.2 | 72.6 | 28.9 | 112.9 | 25.9 |
2 | 88.6 | 22.6 | 80.7 | 9.1 | 90.6 | 19.5 | |
10 | 75.2 | 9.5 | 78.7 | 6.0 | 83.8 | 9.2 | |
Clopidol | 5 | 98.4 | 11.7 | 60.4 | 26.9 | 95.3 | 16.1 |
10 | 110.1 | 20.9 | 74.3 | 22.2 | 98.5 | 11.0 | |
20 | 79.5 | 9.7 | 71.5 | 5.6 | 99.5 | 11.4 | |
Clorsulon | 5 | 70.7 | 9.9 | 71.5 | 8.5 | 113.7 | 6.9 |
10 | 77.3 | 11.3 | 94.8 | 8.3 | 99.0 | 11.5 | |
20 | 78.3 | 5.7 | 81.4 | 20.5 | 103.8 | 9.1 | |
Closantel | 5 | 98.4 | 12.7 | 75.1 | 19.5 | 105.4 | 10.3 |
10 | 92.1 | 4.7 | 90.9 | 5.9 | 101.9 | 9.6 | |
20 | 82.8 | 18.2 | 92.9 | 12.6 | 98.9 | 14.0 | |
Colchicine | 1 | 61.5 | 30.8 | 99.7 | 13.6 | 98.0 | 5.5 |
2 | 70.5 | 12.0 | 81.8 | 4.7 | 95.8 | 15.2 | |
10 | 68.1 | 7.2 | 77.5 | 6.0 | 92.6 | 9.8 | |
Cyproheptadine | 5 | 100.8 | 17.4 | 85.1 | 13.4 | 79.2 | 17.6 |
10 | 91.8 | 11.4 | 76.3 | 22.8 | 96.1 | 30.4 | |
20 | 73.9 | 7.1 | 78.8 | 17.1 | 78.8 | 16.9 | |
Dapsone | 1 | 65.7 | 27.6 | 75.0 | 31.1 | 113.6 | 14.2 |
2 | 61.5 | 9.0 | 77.7 | 9.0 | 96.7 | 6.7 | |
10 | 67.1 | 14.0 | 68.8 | 12.9 | 85.9 | 11.1 | |
Monoacetyl dapsone | 1 | 60.1 | 18.4 | 60.3 | 24.2 | 72.6 | 29.2 |
2 | 68.4 | 17.2 | 80.7 | 22.1 | 91.1 | 18.5 | |
10 | 87.9 | 6.5 | 92.7 | 3.9 | 93.1 | 7.6 | |
Diclazuril | 5 | 98.1 | 12.1 | 67.7 | 6.4 | 119.8 | 2.7 |
10 | 90.5 | 3.8 | 94.0 | 10.5 | 119.3 | 4.4 | |
20 | 91.8 | 9.9 | 96.0 | 4.4 | 116.0 | 2.5 | |
Diethylcarbamazine | 5 | 105.3 | 10.9 | 118.9 | 5.8 | 106.0 | 13.8 |
10 | 84.2 | 10.8 | 98.6 | 6.2 | 105.1 | 19.6 | |
20 | 61.8 | 8.8 | 78.2 | 7.5 | 92.7 | 12.1 | |
Dimetridazole | 1 | 103.1 | 24.6 | 115.1 | 8.7 | 117.7 | 22.9 |
2 | 77.7 | 16.2 | 80.8 | 9.6 | 117.2 | 14.1 | |
10 | 60.5 | 6.2 | 63.1 | 13.3 | 92.1 | 7.4 | |
Diminazene | 5 | 106.9 | 10.6 | 73.5 | 19.6 | 105.0 | 10.2 |
10 | 84.1 | 10.2 | 99.8 | 8.0 | 89.9 | 5.4 | |
20 | 71.0 | 4.8 | 97.4 | 13.8 | 86.4 | 13.2 | |
Diphenhydramine | 5 | 96.2 | 8.0 | 80.1 | 13.4 | 81.2 | 23.7 |
10 | 92.3 | 14.2 | 73.5 | 19.5 | 117.8 | 2.7 | |
20 | 72.7 | 8.1 | 61.9 | 9.2 | 99.1 | 29.2 | |
dl-Methylephedrine HCl | 5 | 82.1 | 9.3 | 117.0 | 9.1 | 96.6 | 9.5 |
10 | 70.8 | 5.9 | 94.9 | 6.0 | 88.9 | 14.2 | |
20 | 60.3 | 6.0 | 75.8 | 10.9 | 89.5 | 10.7 | |
Efrotomycin | 5 | 116.9 | 23.4 | 116.7 | 21.0 | 104.9 | 14.5 |
10 | 101.6 | 11.3 | 107.1 | 17.7 | 104.0 | 24.3 | |
20 | 98.3 | 10.8 | 112.8 | 7.9 | 108.6 | 7.5 | |
Emamectin benzoate | 5 | 94.7 | 10.8 | 67.6 | 5.9 | 109.5 | 4.2 |
10 | 86.6 | 5.9 | 80.0 | 8.7 | 98.9 | 7.2 | |
20 | 83.4 | 6.3 | 83.2 | 8.4 | 93.3 | 7.5 | |
Fluconazole | 5 | 113.9 | 8.3 | 72.5 | 7.5 | 108.6 | 6.9 |
10 | 89.0 | 5.0 | 79.0 | 4.2 | 99.9 | 9.2 | |
20 | 70.2 | 5.1 | 73.4 | 4.5 | 95.9 | 8.6 | |
Halofuginone | 5 | 101.5 | 16.7 | 73.1 | 16.9 | 108.5 | 11.8 |
10 | 78.1 | 14.3 | 87.1 | 9.7 | 90.8 | 11.0 | |
20 | 72.0 | 10.5 | 79.0 | 9.7 | 90.5 | 4.5 | |
Imidocarb | 5 | 83.9 | 19.9 | 115.3 | 11.4 | 104.5 | 19.0 |
10 | 85.1 | 7.8 | 111.6 | 27.0 | 104.5 | 17.2 | |
20 | 80.1 | 5.9 | 118.1 | 7.3 | 90.4 | 30.5 | |
Ipronidazole | 1 | 107.1 | 18.3 | 75.0 | 22.1 | 85.0 | 10.0 |
2 | 95.2 | 10.3 | 64.0 | 4.7 | 107.3 | 22.7 | |
10 | 62.6 | 6.0 | 62.0 | 5.9 | 88.1 | 13.0 | |
Isometamidium | 5 | 97.2 | 8.3 | 89.0 | 10.8 | 98.2 | 7.8 |
10 | 88.9 | 6.8 | 104.2 | 18.8 | 100.5 | 11.3 | |
20 | 87.9 | 15.2 | 105.6 | 2.9 | 108.2 | 8.5 | |
Ketoprofen | 5 | 96.9 | 4.3 | 72.8 | 15.1 | 119.3 | 3.2 |
10 | 88.8 | 8.5 | 87.9 | 9.9 | 110.7 | 9.3 | |
20 | 77.9 | 9.1 | 81.0 | 9.7 | 106.5 | 8.1 | |
Loperamide | 5 | 96.7 | 6.8 | 69.0 | 7.2 | 98.3 | 3.8 |
10 | 82.6 | 3.9 | 78.2 | 8.8 | 90.2 | 6.7 | |
20 | 75.3 | 5.5 | 79.2 | 7.9 | 84.7 | 5.0 | |
Metoclopramide | 5 | 114.5 | 26.1 | 75.4 | 12.1 | 97.9 | 12.3 |
10 | 81.6 | 12.0 | 80.8 | 23.4 | 90.9 | 9.3 | |
20 | 70.3 | 6.6 | 71.7 | 8.1 | 92.0 | 13.3 | |
Metronidazole | 1 | 89.3 | 12.3 | 107.2 | 7.8 | 120.3 | 13.2 |
2 | 70.9 | 18.3 | 66.9 | 9.0 | 105.1 | 8.9 | |
10 | 60.9 | 7.7 | 63.3 | 15.3 | 90.0 | 8.8 | |
Metronidazole-OH | 1 | 68.3 | 6.6 | 111.1 | 11.1 | 97.2 | 12.6 |
2 | 60.0 | 9.2 | 103.3 | 6.6 | 91.9 | 8.3 | |
10 | 63.6 | 6.3 | 104.2 | 10.2 | 85.1 | 11.4 | |
Morantel | 5 | 95.8 | 6.3 | 73.9 | 6.2 | 108.4 | 5.7 |
10 | 85.5 | 9.7 | 83.9 | 3.6 | 107.6 | 16.0 | |
20 | 71.5 | 5.2 | 79.1 | 4.6 | 102.9 | 8.6 | |
Naloxone | 5 | 102.4 | 11.1 | 68.6 | 23.2 | 101.3 | 6.6 |
10 | 80.6 | 4.9 | 79.6 | 7.0 | 100.8 | 8.7 | |
20 | 70.9 | 4.2 | 79.7 | 7.4 | 89.1 | 10.4 | |
Nandrolone | 5 | 99.1 | 9.1 | 75.5 | 10.3 | 106.6 | 7.9 |
10 | 88.3 | 4.5 | 82.5 | 9.5 | 104.6 | 10.6 | |
20 | 75.3 | 5.7 | 83.8 | 6.5 | 94.3 | 5.1 | |
Olaquindox | 1 | 92.5 | 6.2 | 72.1 | 12.1 | 75.7 | 10.7 |
2 | 86.5 | 6.9 | 82.6 | 10.4 | 89.2 | 7.3 | |
10 | 70.5 | 7.5 | 64.7 | 5.1 | 62.7 | 14.0 | |
Oxyclozanide | 5 | 104.3 | 12.9 | 64.2 | 14.9 | 97.8 | 10.7 |
10 | 95.3 | 4.9 | 97.5 | 15.8 | 117.7 | 13.4 | |
20 | 86.9 | 16.4 | 104.3 | 16.0 | 114.9 | 6.6 | |
Phenacetin | 5 | 117.9 | 9.6 | 110.6 | 9.5 | 101.4 | 13.9 |
10 | 88.8 | 8.1 | 95.8 | 6.1 | 99.3 | 13.2 | |
20 | 72.0 | 9.7 | 74.6 | 8.3 | 93.1 | 8.8 | |
Pirlimycin | 5 | 115.2 | 5.5 | 70.8 | 10.2 | 105.3 | 6.5 |
10 | 82.9 | 6.4 | 79.7 | 3.9 | 94.9 | 5.7 | |
20 | 72.9 | 5.2 | 74.7 | 3.8 | 90.7 | 5.5 | |
Pyrmethamine | 1 | 79.9 | 5.5 | 67.9 | 12.0 | 87.8 | 4.0 |
2 | 80.6 | 7.9 | 69.5 | 8.6 | 85.5 | 9.5 | |
10 | 69.8 | 4.6 | 75.3 | 8.6 | 77.5 | 6.0 | |
Ractopamine | 5 | 87.8 | 7.6 | 60.5 | 12.3 | 94.4 | 2.9 |
10 | 81.9 | 5.6 | 79.6 | 3.4 | 91.8 | 7.5 | |
20 | 72.6 | 6.6 | 75.0 | 4.1 | 88.6 | 6.5 | |
Robenidine | 5 | 98.0 | 13.9 | 67.9 | 17.8 | 80.1 | 12.5 |
10 | 71.7 | 12.7 | 74.7 | 4.8 | 77.3 | 16.1 | |
20 | 82.3 | 20.3 | 70.2 | 15.1 | 72.2 | 17.8 | |
Roxarsone | 5 | 118.0 | 22.4 | 111.2 | 18.9 | 111.5 | 8.5 |
10 | 82.5 | 7.0 | 76.4 | 10.9 | 103.9 | 29.6 | |
20 | 61.7 | 6.1 | 72.4 | 4.2 | 113.8 | 14.9 | |
Scopolamine | 5 | 97.1 | 6.9 | 61.6 | 11.6 | 111.8 | 11.7 |
10 | 82.1 | 4.9 | 74.1 | 11.5 | 97.1 | 11.6 | |
20 | 70.5 | 10.5 | 79.4 | 7.8 | 89.0 | 6.9 | |
Succinyl-sulfathiazole | 5 | 75.8 | 17.1 | 78.3 | 20.2 | 89.2 | 24.3 |
10 | 77.4 | 10.0 | 77.5 | 6.2 | 80.3 | 15.3 | |
20 | 70.6 | 13.3 | 80.5 | 8.9 | 81.5 | 9.7 | |
Sulfabendzamide | 5 | 112.3 | 4.6 | 66.1 | 10.7 | 111.5 | 4.3 |
10 | 87.0 | 9.1 | 74.8 | 8.5 | 101.6 | 11.9 | |
20 | 70.1 | 7.2 | 71.2 | 9.2 | 97.4 | 8.6 | |
Sulfameter | 5 | 105.5 | 11.8 | 62.6 | 11.2 | 111.5 | 4.3 |
10 | 81.8 | 11.5 | 72.7 | 8.7 | 101.6 | 11.9 | |
20 | 70.4 | 14.2 | 69.8 | 9.3 | 97.4 | 8.6 | |
Sulfamoxol | 5 | 112.2 | 8.0 | 73.6 | 18.4 | 101.5 | 6.8 |
10 | 78.9 | 6.8 | 80.8 | 6.1 | 102.8 | 3.6 | |
20 | 70.7 | 7.5 | 78.1 | 17.0 | 98.8 | 7.1 | |
Sulfapyridine | 5 | 115.3 | 7.9 | 113.5 | 4.6 | 110.9 | 7.1 |
10 | 91.7 | 9.5 | 94.1 | 9.1 | 100.3 | 7.4 | |
20 | 71.4 | 4.6 | 74.1 | 6.7 | 93.8 | 7.7 | |
Sulfisomidine | 5 | 86.6 | 15.2 | 113.7 | 6.6 | 115.9 | 3.7 |
10 | 73.8 | 12.1 | 94.0 | 3.6 | 100.1 | 13.7 | |
20 | 63.1 | 5.3 | 72.9 | 10.2 | 96.5 | 5.4 | |
Thiacloprid | 5 | 96.6 | 4.7 | 71.8 | 8.3 | 108.1 | 5.1 |
10 | 83.5 | 6.2 | 78.3 | 6.4 | 102.2 | 10.3 | |
20 | 70.4 | 5.3 | 72.1 | 8.0 | 98.8 | 9.9 | |
Tinidazole | 5 | 117.0 | 8.6 | 72.2 | 23.0 | 104.6 | 7.1 |
10 | 92.2 | 8.8 | 83.0 | 8.5 | 98.3 | 7.0 | |
20 | 70.8 | 9.9 | 73.0 | 3.0 | 92.2 | 10.5 | |
Toltrazuril | 5 | 78.8 | 11.2 | 79.1 | 17.5 | 99.2 | 14.6 |
10 | 91.4 | 5.6 | 107.6 | 3.1 | 111.8 | 11.6 | |
20 | 90.3 | 7.5 | 114.3 | 4.9 | 118.9 | 7.2 | |
Tripelennamine | 5 | 110.5 | 20.9 | 81.9 | 21.1 | 110.0 | 11.9 |
10 | 115.7 | 9.6 | 69.9 | 12.8 | 115.9 | 21.4 | |
20 | 83.0 | 14.0 | 73.6 | 14.3 | 97.2 | 18.0 | |
Valnemulin | 5 | 87.9 | 9.7 | 60.9 | 8.4 | 94.3 | 5.8 |
10 | 79.7 | 5.1 | 72.6 | 7.3 | 84.1 | 7.4 | |
20 | 75.3 | 5.6 | 74.0 | 7.0 | 80.0 | 5.1 | |
Yohimbine | 5 | 111.9 | 8.3 | 71.4 | 19.9 | 88.8 | 4.3 |
10 | 81.3 | 6.9 | 78.8 | 4.8 | 87.7 | 11.7 | |
20 | 70.2 | 8.7 | 77.3 | 9.9 | 89.2 | 8.9 | |
Zilpaterol | 5 | 96.1 | 6.8 | 73.8 | 9.2 | 113.5 | 6.5 |
10 | 80.4 | 8.2 | 83.2 | 7.9 | 105.6 | 10.3 | |
20 | 70.0 | 7.0 | 75.2 | 5.9 | 98.9 | 10.3 | |