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Table 1 Kinetic parameters of mitochondrial complexes

From: Steady-state kinetic analysis of mitochondrial respiratory enzymes from bovine heart mitochondria

 

Varied substrates

Vmax

Vmax unit

Km

Km unit

Indirect kcat(s−1)

NADH: Ubiquinone oxidoreductase

NADH

0.2909 ± 0.0405

μM·s−1

11.2397 ± 5.7066

μM

0.2644 ± 0.0368*

UQ

0.3667

(95% CI 0.3048–0.4640)

12.5676

(95% CI 5.9611–25.9138)

0.3334*

(95% CI 0.2771–0.4218)

Succinate dehydrogenase

Succinate

0.3197 ± 0.0759

48.5691 ± 22.5309α

0.2459 ± 0.0584*

12.1423 ± 5.6327β,*

mM

DUQ

0.2095

(95% CI 0.1731–0.2666)

9.7331α

(95% CI 4.2749–21.0149)

μM

0.1612*

(95% CI 0.1332–0.2051)

6.0832γ,*

(95% CI 2.6718–13.1343)

Coenzyme Q: cytochrome c oxidoreductase

DUQH2

0.1827 ± 0.0287

5.1969 ± 2.4045

0.0609 ± 0.0096*

Oxidized Cytc

0.2071

(95% CI 0.1601–0.2893)

37.8970

(95% CI 17.9899–79.0282)

0.0690*

(95% CI 0.0534–0.0964)

Cytochrome c oxidase

Reduced Cytc

1.4380 ± 0.4977

126.7201 ± 69.9096

0.2146 ± 0.0743*

  1. *Theoretically calculated value, αKm of DCPIP, βKm of Succinate, γKm of Decylubiquinone