Fig. 1

Effect of glycolaldehyde on RAGE and ROS production and lipid accumulation in 3T3-L1 cells. A 3T3-L1 cells were stimulated with MDI in the presence or absence of glycolaldehyde (GA; 0.1, 1, and 10 μM) for 2 h. Cell lysates were collected and subjected to western blotting using antibodies against the target gene RAGE. B ROS level was determined as described in materials and methods. Adipocytes were treated with DCF-DA (10 μM) in the presence or absence of GA (0.1, 1, and 10 μM) for 30 min (Glycolaldehyde; GLY). C, D Confluent 3T3-L1 cells were treated with MDI containing GA (0.1, 1, and 10 μM) at the early phases of adipocyte differentiation. Adipocytes were then stained with Oil Red O on day 7. RAGE level was measure by western blotting with β-actin as internal control. Data presented as mean ± SEM. *p < 0.05 vs. MDI treatment