Fig. 6

Inhibition of GA-induced adipogenesis and insulin resistance. Effects of RAGE and ROS on inflammatory signaling by GA in adipocytes. 3T3-L1 cells were transfected with control siRNA and specific RAGE siRNA and then treated with GA to check the knockdown of RAGE. A 3T3-L1 cells were transfected with siRAGE and then stimulated with GA (10 μM) for 24 h. Expression of PPARγ, p-c/EBPβ, TNF-α, IRS-1, and GLUT4 protein was determined by western blot assay. B 3T3-L1 cells were incubated with the ROS inhibitor NAC for 2 h and then stimulated with GA for 24 h. Whole cell lysates were analyzed by western blot assay. β-actin protein levels were used as an internal control. Data are expressed as the mean ± SEM of three independent experiments. *p < 0.05, significantly different from the untreated group