Table 1 Primer sequences
From: Glycolaldehyde disrupts insulin signaling and glucose uptake through adipogenesis
Gene | Forward primer (5’ → 3’) | Reverse primer (5’ → 3’) |
|---|---|---|
PPARγ | GCC CTT TGG TGA CTT TAT GGA | GCA GCA GGT TGT CTT GGA TG |
c/EBPα | GCG AGC ACG AGA CGT CTA TAG | GCC AGG AAC TCG TCG TTG AA |
TNF-α | CCC TCA CAC TCA GAT CAT CTT CT | GCT ACG ACG TGG GCT ACA G |
IL-6 | CCA CGG CCT TCC CTA CTT C | TTG GGA GTG GTA TCC TCT GTG A |
aP2 | GAA CCT GGA AGC TTG TCT CCA GTG | GAT GCT CTT CAC CTT CCT GTC GTC TGC |
LPL | TTG CGC CTC CTG CTC AA CCC | CCC CTC CTC GGA AGG CGG TC |
FAS | CTG AGA TCC CAG CAC TTC TTG | GCC TCC GAA GCC AAA TGA |
SREBP-1c | GGC TAT TCC GTG AAC ATC TCC TA | ATC CAA GGG CAT CTG AGA ACT |
GAPDH | TGC ATC CTG CAC CAC CAA | TCC ACG ATG CCA AAG TTG TC |