Validation of an optimized HPLC–UV method for the quantification of formononetin and biochanin A in Trifolium pratense extract

Applied Biological Chemistry

Table 1 Accuracy of formononetin (1) and biochanin A (2) quantification

Compound	Concentration (mg/mL)	Measured content (mg/g)					Average recovery rate (%)	Overall recovery rate (%)
Compound	Concentration (mg/mL)	1st	2nd	3rd	4th	5th	Average recovery rate (%)	Overall recovery rate (%)
1	0.125	86.86	90.30	88.68	86.75	86.62	87.84	88.38
	0.0625	84.81	87.25	84.38	84.29	82.15	84.58
	0.03125	91.52	91.73	91.45	88.80	85.73	89.85
	0.015625	91.77	95.59	92.05	90.27	90.66	91.27
2	0.125	106.22	106.38	106.49	106.52	106.19	106.36	101.63
	0.0625	98.54	99.02	98.50	100.37	100.74	99.63
	0.03125	100.45	100.02	100.40	100.04	100.21	100.22
	0.015625	100.18	100.52	100.92	100.69	99.11	100.28